Detailed information of TA system TA148677

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2819271..2820115
• Replicon: chromosome
• Accession: NZ_CP048010
• Organism: Escherichia coli strain RUT3575

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: GW576_RS13915
• Protein ID: WP_000854686.1
• Coordinates: 2819271..2819654 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: GW576_RS13920
• Protein ID: WP_001285602.1
• Coordinates: 2819735..2820115 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW576_RS13875	2814274..2814765	-	492	WP_001297187.1	DUF1097 domain-containing protein	-
GW576_RS13880	2814867..2815421	-	555	WP_001001902.1	molecular chaperone YcdY	-
GW576_RS13885	2815445..2816182	-	738	WP_000283664.1	zinc-binding phosphatase	-
GW576_RS13890	2816237..2817175	-	939	WP_000351311.1	glyoxylate/hydroxypyruvate reductase GhrA	-
GW576_RS13900	2817646..2818487	-	842	Protein_2656	DUF4942 domain-containing protein	-
GW576_RS13905	2818572..2818769	-	198	WP_000839253.1	DUF957 domain-containing protein	-
GW576_RS13910	2818786..2819274	-	489	WP_032180032.1	hypothetical protein	-
GW576_RS13915	2819271..2819654	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
GW576_RS13920	2819735..2820115	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
GW576_RS13925	2820126..2820809	-	684	WP_000086768.1	hypothetical protein	-
GW576_RS13930	2820828..2821049	-	222	WP_000692298.1	DUF987 domain-containing protein	-
GW576_RS13935	2821184..2821498	-	315	Protein_2663	antirestriction protein	-
GW576_RS13940	2821590..2822411	-	822	WP_001761104.1	DUF945 domain-containing protein	-
GW576_RS13945	2822512..2822720	-	209	Protein_2665	DUF905 family protein	-
GW576_RS13950	2822821..2823276	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T148677 WP_000854686.1 NZ_CP048010:c2819654-2819271 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T148677 NZ_CP063841:37266-37535 [Sphingopyxis granuli]
TTGGCCTGGACAATTGAACTTTCCGGCGCCGCCGAAAAGACACTGAAGAAGCTGGACCGACAAGCAGCTAAGCGTATCAC
CAGCTTTCTTCGCGAGCGGGTTGCCGATGGCGAAGGCCCAAGGTCGACGGGCAAAGCGCTTCAAGGTCCGCTTGCGGGCT
TATGGCGCTATCGTGTCGGCGATTTTCGGATCATCTGCGAAATCGAAGATGGAAAACTGGTGGTCCTTGTCGTTACCATA
GGACATCGCGGCGATGTTTACCGCCGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT148677 WP_001285602.1 NZ_CP048010:c2820115-2819735 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT148677 NZ_CP063841:37055-37282 [Sphingopyxis granuli]
ATGGCAACGTCGGTAAGACTCGATGCCGAAACCGAGGGGCGGCTTGATCGGCTGGCCGACCTGACCGGTCGGACCAAAGC
CTATTACATCCGCGAGATGATCGAGCGCAGCATCGAGGACATGGAAGACTATTACCTTGCCGCTAAGGTTGTCGAAGACA
TTCGGGCGGGCCGCGAAACCGTCTCCAGCCTCGACGACGTGGAGCGCCGACTTGGCCTGGACAATTGA