Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1351921..1352546 Replicon chromosome
Accession NZ_CP048010
Organism Escherichia coli strain RUT3575

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag GW576_RS06865 Protein ID WP_000911330.1
Coordinates 1352148..1352546 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag GW576_RS06860 Protein ID WP_000450524.1
Coordinates 1351921..1352148 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW576_RS06835 1347724..1348194 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
GW576_RS06840 1348194..1348766 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
GW576_RS06845 1348912..1349790 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
GW576_RS06850 1349807..1350841 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
GW576_RS06855 1351054..1351767 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
GW576_RS06860 1351921..1352148 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
GW576_RS06865 1352148..1352546 + 399 WP_000911330.1 tRNA(fMet)-specific endonuclease VapC Toxin
GW576_RS06870 1352693..1353556 + 864 WP_001267507.1 neutral zinc metallopeptidase -
GW576_RS06875 1353571..1355586 + 2016 WP_000829323.1 tRNA cytosine(34) acetyltransferase TmcA -
GW576_RS06880 1355660..1356001 + 342 Protein_1277 esterase -
GW576_RS06885 1356083..1356829 - 747 WP_053906593.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T148666 WP_000911330.1 NZ_CP048010:1352148-1352546 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T148666 NZ_CP063835:37249-37407 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT148666 WP_000450524.1 NZ_CP048010:1351921-1352148 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT148666 NZ_CP063835:37138-37203 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References