Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 347636..348436 Replicon chromosome
Accession NZ_CP048010
Organism Escherichia coli strain RUT3575

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag GW576_RS01945 Protein ID WP_000342449.1
Coordinates 347909..348436 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GW576_RS01940 Protein ID WP_001277108.1
Coordinates 347636..347902 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW576_RS01920 343293..343961 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
GW576_RS01925 343954..345012 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
GW576_RS01930 345257..346111 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GW576_RS01935 346383..347486 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GW576_RS01940 347636..347902 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
GW576_RS01945 347909..348436 + 528 WP_000342449.1 GNAT family N-acetyltransferase Toxin
GW576_RS01950 348433..348816 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
GW576_RS01955 349240..350349 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GW576_RS01960 350397..351323 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GW576_RS01965 351320..352597 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
GW576_RS01970 352594..353361 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T148660 WP_000342449.1 NZ_CP048010:347909-348436 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T148660 NZ_CP063833:c4831107-4830823 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT148660 WP_001277108.1 NZ_CP048010:347636-347902 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT148660 NZ_CP063833:c4831339-4831097 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References