Detailed information of TA system TA148658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 1659773..1660582
• Replicon: chromosome
• Accession: NZ_CP048008
• Organism: Staphylococcus lugdunensis strain CGMH-SL118

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: -
• Locus tag: GWC79_RS08200
• Protein ID: WP_002458758.1
• Coordinates: 1660412..1660582 (+)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: A0A853V5R2
• Locus tag: GWC79_RS08195
• Protein ID: WP_002458757.1
• Coordinates: 1659773..1660372 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWC79_RS08175	1655360..1656817	+	1458	WP_002477974.1	ABC transporter substrate-binding protein/permease	-
GWC79_RS08180	1656810..1657535	+	726	WP_002477975.1	amino acid ABC transporter ATP-binding protein	-
GWC79_RS08185	1657993..1659135	+	1143	WP_141760444.1	tRNA epoxyqueuosine(34) reductase QueG	-
GWC79_RS08190	1659137..1659607	+	471	WP_002458756.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
GWC79_RS08195	1659773..1660372	+	600	WP_002458757.1	glucosamine-6-phosphate isomerase	Antitoxin
GWC79_RS08200	1660412..1660582	+	171	WP_002458758.1	SAS053 family protein	Toxin
GWC79_RS08205	1660756..1661151	+	396	WP_002458759.1	hypothetical protein	-
GWC79_RS08210	1661279..1662664	+	1386	WP_002477977.1	class II fumarate hydratase	-
GWC79_RS08215	1662729..1663565	-	837	WP_228865234.1	RluA family pseudouridine synthase	-
GWC79_RS08220	1663733..1664845	+	1113	WP_002477979.1	GAF domain-containing sensor histidine kinase	-
GWC79_RS08225	1664867..1665490	+	624	WP_002477980.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (131-195)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6565.10 Da
• Isoelectric Point: 4.6723

>T148658 WP_002458758.1 NZ_CP048008:1660412-1660582 [Staphylococcus lugdunensis]
MSKEKKSRLNYHEEENAMVENLEDLKELGKEMEHISEKNDEEKVNQSHDTSKSSNS

Nucleotide

• Length: 171 bp

>T148658 NZ_CP063833:4113154-4113372 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22619.51 Da
• Isoelectric Point: 4.9539

>AT148658 WP_002458757.1 NZ_CP048008:1659773-1660372 [Staphylococcus lugdunensis]
MAMNFKVFENKDQVAEYSADIIRKQFNNNPTTIAGFHLNKEASPVLDMLKKNVDQHAVDFSQINILDYDQNQEYFKALGV
PESQIYPISYDQDAEAFISDKIKTKDNKGKLILQVASIDDQGNLNISLRQGLLNAREIILVITGHNKKEVVEKLYNENGK
SNFEPADLKAHRMVTVILDEAAAEGLPEDVRHYFTDRFA

Nucleotide

• Length: 600 bp

>AT148658 NZ_CP063833:4112753-4113127 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A853V5R2