Detailed information of TA system TA148655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 1363857..1364375
• Replicon: chromosome
• Accession: NZ_CP048008
• Organism: Staphylococcus lugdunensis strain CGMH-SL118

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: -
• Locus tag: GWC79_RS06490
• Protein ID: WP_002478973.1
• Coordinates: 1364163..1364375 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: A0A853V130
• Locus tag: GWC79_RS06485
• Protein ID: WP_002492098.1
• Coordinates: 1363857..1364150 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWC79_RS06465	1359457..1360095	+	639	WP_002478970.1	thiamine phosphate synthase	-
GWC79_RS06470	1360185..1361060	+	876	WP_002461212.1	membrane protein insertase YidC	-
GWC79_RS06475	1361444..1362085	-	642	WP_002478971.1	HD domain-containing protein	-
GWC79_RS06480	1362207..1363691	-	1485	WP_002478972.1	cardiolipin synthase	-
GWC79_RS06485	1363857..1364150	+	294	WP_002492098.1	copper-sensing transcriptional repressor CsoR	Antitoxin
GWC79_RS06490	1364163..1364375	+	213	WP_002478973.1	heavy metal-associated domain-containing protein	Toxin
GWC79_RS06495	1364466..1364603	+	138	WP_012990785.1	Lmo0850 family protein	-
GWC79_RS06500	1365017..1366207	-	1191	WP_002461205.1	FtsW/RodA/SpoVE family cell cycle protein	-
GWC79_RS06505	1366563..1367633	+	1071	WP_002461204.1	D-alanine--D-alanine ligase	-
GWC79_RS06510	1367651..1369009	+	1359	WP_002478974.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (19-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-90)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8070.03 Da
• Isoelectric Point: 3.9947

>T148655 WP_002478973.1 NZ_CP048008:1364163-1364375 [Staphylococcus lugdunensis]
VEKRLIYITNVTSEDDLENLRTRLLEMIGVNEVVINFDERAVNVTFETPTNLNALEKEIYDAGYHAIDDL

Nucleotide

• Length: 213 bp

>T148655 NZ_CP063833:879576-879983 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11177.85 Da
• Isoelectric Point: 6.6367

>AT148655 WP_002492098.1 NZ_CP048008:1363857-1364150 [Staphylococcus lugdunensis]
MNEQDHAHHSKIVKANLKSRLNRIEGQVRAINRMVEDDVYCDDVLTQIRATRSALTSVATKLLEHHMKSCIMDKINDGQE
VEAMEELLVTFQKMLKD

Nucleotide

• Length: 294 bp

>AT148655 NZ_CP063833:879329-879595 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A853V130