Detailed information of TA system TA148653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /HTH_XRE(antitoxin)
• Location: 11715..12520
• Replicon: chromosome
• Accession: NZ_CP048008
• Organism: Staphylococcus lugdunensis strain CGMH-SL118

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GWC79_RS00155
• Protein ID: WP_228865373.1
• Coordinates: 12056..12520 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GWC79_RS00150
• Protein ID: WP_228865372.1
• Coordinates: 11715..12044 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWC79_RS00100	6991..7476	-	486	WP_228865549.1	siphovirus Gp157 family protein	-
GWC79_RS00105	7949..8332	+	384	WP_002478859.1	hypothetical protein	-
GWC79_RS00110	8280..8591	-	312	WP_070866275.1	hypothetical protein	-
GWC79_RS00115	8631..9125	-	495	WP_228865367.1	ORF6C domain-containing protein	-
GWC79_RS00120	9185..9415	+	231	WP_051522842.1	hypothetical protein	-
GWC79_RS00125	9412..9558	-	147	WP_183998736.1	hypothetical protein	-
GWC79_RS00130	9571..10452	-	882	WP_228865369.1	DUF3102 domain-containing protein	-
GWC79_RS00135	10513..11115	+	603	WP_228865370.1	hypothetical protein	-
GWC79_RS00140	11125..11268	-	144	WP_002478861.1	hypothetical protein	-
GWC79_RS00145	11284..11523	-	240	WP_228865371.1	helix-turn-helix transcriptional regulator	-
GWC79_RS00150	11715..12044	+	330	WP_228865372.1	helix-turn-helix transcriptional regulator	Antitoxin
GWC79_RS00155	12056..12520	+	465	WP_228865373.1	ImmA/IrrE family metallo-endopeptidase	Toxin
GWC79_RS00160	12547..12729	+	183	WP_012991013.1	hypothetical protein	-
GWC79_RS00165	12733..13701	+	969	WP_062078527.1	DUF3644 domain-containing protein	-
GWC79_RS00170	13883..15265	+	1383	WP_228865374.1	recombinase family protein	-
GWC79_RS00175	15362..15988	-	627	WP_228865324.1	hypothetical protein	-
GWC79_RS00180	15985..16260	-	276	WP_014533277.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	127..54451	54324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (28-119)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-65)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 18348.92 Da
• Isoelectric Point: 6.0754

>T148653 WP_228865373.1 NZ_CP048008:12056-12520 [Staphylococcus lugdunensis]
MGLYEEICIDNDMVEIKETNKLPSFQSGFYMNGTVYIKSDLSEKEKIKVLYEELAHHKLTYGNILDQSKWINRKFENYAR
RHGYEAALPLRIIIEAHHFGVSNLYELAQYVQLSEEYVLEILEHYKQKHGIGIHYGEYSITFEPLRVFRLYEVY

Nucleotide

• Length: 465 bp

>T148653 NZ_CP063833:355289-355636 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12653.23 Da
• Isoelectric Point: 4.9783

>AT148653 WP_228865372.1 NZ_CP048008:11715-12044 [Staphylococcus lugdunensis]
MRTNDEIITIIKTTLKEQNMSLSELARRVGMAKSAVSRYLNLTREFPLNRAEDFANALHISTEYLLGFNGSEQTRQQDTM
AAHFDKEGLTEEEIEEVNKFVEWVKNRDK

Nucleotide

• Length: 330 bp

>AT148653 NZ_CP063833:355636-355935 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA