Detailed information of TA system TA148651

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 1097786..1098321
• Replicon: chromosome
• Accession: NZ_CP048007
• Organism: Staphylococcus lugdunensis strain CGMH-SL131

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: C5QLS6
• Locus tag: GWC80_RS05110
• Protein ID: WP_002445368.1
• Coordinates: 1097998..1098321 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: C5QLS5
• Locus tag: GWC80_RS05105
• Protein ID: WP_001058489.1
• Coordinates: 1097786..1097995 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWC80_RS05070	1093042..1093326	+	285	WP_002461410.1	co-chaperone GroES	-
GWC80_RS05075	1093386..1095008	+	1623	WP_002461409.1	chaperonin GroEL	-
GWC80_RS05080	1095077..1096249	-	1173	WP_002492309.1	tyrosine-type recombinase/integrase	-
GWC80_RS05085	1096263..1096937	-	675	WP_002445345.1	helix-turn-helix transcriptional regulator	-
GWC80_RS05090	1097110..1097328	+	219	WP_000153640.1	helix-turn-helix transcriptional regulator	-
GWC80_RS05095	1097333..1097650	+	318	WP_002445364.1	helix-turn-helix domain-containing protein	-
GWC80_RS05100	1097647..1097793	+	147	WP_002445365.1	hypothetical protein	-
GWC80_RS05105	1097786..1097995	+	210	WP_001058489.1	hypothetical protein	Antitoxin
GWC80_RS05110	1097998..1098321	+	324	WP_002445368.1	DUF1474 family protein	Toxin
GWC80_RS05115	1098385..1099254	+	870	WP_002445370.1	primase alpha helix C-terminal domain-containing protein	-
GWC80_RS05120	1099268..1100977	+	1710	WP_002445371.1	DUF927 domain-containing protein	-
GWC80_RS05125	1101325..1101690	+	366	WP_002445372.1	hypothetical protein	-
GWC80_RS05130	1101704..1102345	+	642	WP_002492310.1	pathogenicity island protein	-
GWC80_RS05135	1102633..1102977	+	345	WP_094449183.1	pathogenicity island protein	-
GWC80_RS05140	1102991..1103224	+	234	WP_002492317.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1093042..1111319	18277

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 13107.51 Da
• Isoelectric Point: 4.8571

>T148651 WP_002445368.1 NZ_CP048007:1097998-1098321 [Staphylococcus lugdunensis]
MNWEIKNLFSDLKLLKDSFEDLKDNHGWHFEELYPHEPNHNLNKDELIREGFSYHERRIHNNQMFDLFHLYIEQFDKIIQ
KFHELEKASSENFGEESDDAQKLKITE

Nucleotide

• Length: 324 bp

>T148651 NZ_CP063832:5100699-5100881 [Pseudomonas entomophila]
ATGCGCAGCAGGGACGTGATTCAGCTCATCGAGGCCGATGGCTGGTACGAAGTCGAGGTGAAGGGCAGCCATCATCAGTT
CAGGCACCCGTTCAAGCGGGGGCGGGTCACGGTGCCGCATCCCAAGCGCGATCTACCCAGAGGCACCGCCCACAGCATCC
TCAAACAGGCGGGCTTGAAATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8225.24 Da
• Isoelectric Point: 4.2820

>AT148651 WP_001058489.1 NZ_CP048007:1097786-1097995 [Staphylococcus lugdunensis]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVDGIEVVSEVSREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT148651 NZ_CP063832:5100938-5101345 [Pseudomonas entomophila]
ATGAAATTTCCAGTTGTGCTGCACAAGGACGAGGGCTCGGACTACGGCGTGATCGTGCCCGACGTACCTGGCTGCTTTTC
CGCCGGCGCCAGCGTCTCGCAAGCGCTGGACAACGTCCAGGAGGCTCTGGCGCTGCATTTCGAAGGGCTGGTGGCCGATG
GCGAGGCACTGCCCCAGGCCCAGGAAGTGGACGTGCATGTGGCGAACCCCGACTACGCCGGCGGCGTCTGGGCGGTGGTG
GAGTTCGACGTCACTCCGTACCTGGGCAAGGCGGTACGCTTCAACGCGACGCTGCCGGAGAACCTGCTGCAGCGGATCGA
CGAGAAGGTCAAGCGTGACCATCGCTACGCCTCGCGCTCGGGTTTTCTTGCTTCGGCGGCGCTGCGCGAGCTGGCCGTGG
CGCATTGA

Structures

Toxin

• AlphaFold DB: A0A853V3H1

Antitoxin

• AlphaFold DB: A0A853V601