Detailed information of TA system TA148646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3155173..3156090
• Replicon: chromosome
• Accession: NZ_CP048002
• Organism: Bacillus velezensis strain CACC 316

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: GWK37_RS15835
• Protein ID: WP_007407256.1
• Coordinates: 3155344..3156090 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: GWK37_RS15830
• Protein ID: WP_003154807.1
• Coordinates: 3155173..3155343 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK37_RS15795	3150397..3152028	+	1632	WP_115940866.1	hypothetical protein	-
GWK37_RS15800	3152041..3152412	+	372	WP_115940865.1	hypothetical protein	-
GWK37_RS15805	3152417..3152614	+	198	WP_007610833.1	XkdX family protein	-
GWK37_RS15810	3152671..3153432	+	762	WP_115940864.1	phage portal protein	-
GWK37_RS15815	3153484..3153747	+	264	WP_100261725.1	hemolysin XhlA family protein	-
GWK37_RS15820	3153761..3154024	+	264	WP_003154813.1	phage holin	-
GWK37_RS15825	3154038..3154916	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
GWK37_RS15830	3155173..3155343	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
GWK37_RS15835	3155344..3156090	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
GWK37_RS15840	3156195..3157193	-	999	WP_003154805.1	inorganic phosphate transporter	-
GWK37_RS15845	3157206..3157823	-	618	WP_003154804.1	DUF47 domain-containing protein	-
GWK37_RS15850	3158109..3159425	-	1317	WP_007610842.1	amino acid permease	-
GWK37_RS15855	3159747..3160697	+	951	WP_022553472.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3107661..3192955	85294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T148646 WP_007407256.1 NZ_CP048002:c3156090-3155344 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T148646 NZ_CP063815:2771207-2771656 [Microbacterium sp. A18JL241]
ATGACCCTCGTCGTTCCCGACGTGAACGTGCTCGTTTACGCCTTCCGCGCGGACTCGCCCGATCACGACGTCTACGCCGA
CTGGCTCAATGGCGCTATGCGTGCAGGTCGAATCGGCGTCGCCGACACGATTCTCAGCGGCTTCGTGCGCATCGTGACGC
ACCCCCGCATCTACGCGGAGCCGACTCCAGTCGCCGCGGCGCTGGCCTATGTACGACACCTGATCGACGCTCCGAACACC
GACTGGCTGCGCCAGGGCCCGTCTGCATGGGAGAAGTTCGACGCGCTGGCATCCGAAGACGCCGCGGTGCGCGGCAATCT
CGTGCCGGACGCCCACATCGCCGCGCTCTGTCTTGCCAATGGCGCACGCCTGGCGACGCGGGATCGCGGCTTCGCGCGGT
TCCCCGGATTGAGATTGTTCGATCCGGCGAAGGCGGATGCTGGGCGCTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT148646 WP_003154807.1 NZ_CP048002:c3155343-3155173 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT148646 NZ_CP063815:2770974-2771210 [Microbacterium sp. A18JL241]
ATGCGAACGACACTCAACCTCTCCGACGCCCTCGCGCGCGAGGCGAAGTCGCGCGCTGCGCGTGAGGGACGCACCTTCAC
GAGCTTCCTCGAAGAGGCGCTCCGCGAGCATCTCGCGCGCGAGCTGCCGGATGCGCCTCAGACGCCCCTCCCCACCTACA
CGCCGAAGGTTCCTGGCAGTCTCGTCGACCTCGACGACAAGGACGCGGTCTGGGACGCGCTGGACAAGAGCGCATGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14