Detailed information of TA system TA148645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2342747..2343384
• Replicon: chromosome
• Accession: NZ_CP048002
• Organism: Bacillus velezensis strain CACC 316

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: GWK37_RS11595
• Protein ID: WP_003156187.1
• Coordinates: 2343034..2343384 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: GWK37_RS11590
• Protein ID: WP_003156188.1
• Coordinates: 2342747..2343028 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK37_RS11570	2339112..2339711	-	600	WP_053573675.1	rhomboid family intramembrane serine protease	-
GWK37_RS11575	2339804..2340169	+	366	WP_014304402.1	holo-ACP synthase	-
GWK37_RS11580	2340334..2341341	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
GWK37_RS11585	2341458..2342627	+	1170	WP_015416873.1	alanine racemase	-
GWK37_RS11590	2342747..2343028	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
GWK37_RS11595	2343034..2343384	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
GWK37_RS11600	2343502..2344323	+	822	WP_015416874.1	STAS domain-containing protein	-
GWK37_RS11605	2344328..2344693	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
GWK37_RS11610	2344696..2345097	+	402	WP_003156178.1	anti-sigma regulatory factor	-
GWK37_RS11615	2345109..2346116	+	1008	WP_007609589.1	PP2C family protein-serine/threonine phosphatase	-
GWK37_RS11620	2346180..2346509	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
GWK37_RS11625	2346506..2346988	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
GWK37_RS11630	2346954..2347742	+	789	WP_029974100.1	RNA polymerase sigma factor SigB	-
GWK37_RS11635	2347742..2348344	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T148645 WP_003156187.1 NZ_CP048002:2343034-2343384 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T148645 NZ_CP063814:2722096-2722488 [Microbacterium sp. A18JL200]
ATCGCCGTCGACACCTCGGCGCTCATGGCGATCGTGCTCGGCGAGCGCGACGCCCTCCCCTTCGCCCGCGCACTCGCTGC
GAACACCGGCGACGCGTGTGTCAGCGCTGCGACGCTGGTCGAGGCCACGATCGTCGCCGAAGCACGCCAGGGCAGTGCTG
CCGCGCAAGACCTCGCGGCCGTGCTGCGGGAGACGCGCATCCGCATCGTTCCGCTCGATGCCACGCAGGCGTCGATCGCG
TCGGCCGCGTGGCGCCGCTTCGGCAAAGGGCGCCATACCGCCGCACTCAACCTTGGCGACTGCTACTCCTACGCGCTCGC
TCGACACTTGGGCGCCCCGCTGCTTTTCAAGGGCGACGGCTTCAGGCAGACCGATATCGCGCCGGTCCTCTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT148645 WP_003156188.1 NZ_CP048002:2342747-2343028 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT148645 NZ_CP063814:2721839-2722093 [Microbacterium sp. A18JL200]
ATGGCGCTCAACATCAAAGACCCGGAGGCCGACCGCCTCGCCCGGGCGCTCGCCGCCGCCACCGGTGAGAGCATCACCGT
CGCCGCACGCGCCGCGTTCGCCGAGCGCCTTGCACGGGTACAGGCGCGAGACACCGCGCCCGAGGTGCTCGCCGAGCTCG
AGAGCATCATCGCGCGAGCGCGAGAACGCGAGACGCTCGACGACCGAACCACGGATGAGATCCTCGGCTACGACGAAGCC
GGACTGCCCACGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5