Detailed information of TA system TA148644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 1608653..1609533
• Replicon: chromosome
• Accession: NZ_CP048001
• Organism: Bifidobacterium longum strain CACC 517

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8APN2
• Locus tag: GTQ89_RS06715
• Protein ID: WP_007052625.1
• Coordinates: 1608653..1609144 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8APN1
• Locus tag: GTQ89_RS06720
• Protein ID: WP_007057212.1
• Coordinates: 1609141..1609533 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTQ89_RS06695	1603735..1604967	+	1233	WP_007056207.1	sensor histidine kinase	-
GTQ89_RS06700	1605002..1605727	+	726	WP_007056206.1	response regulator transcription factor	-
GTQ89_RS06705	1605767..1606408	+	642	WP_007052627.1	GNAT family N-acetyltransferase	-
GTQ89_RS06710	1606498..1608603	+	2106	WP_161519591.1	DUF262 domain-containing protein	-
GTQ89_RS06715	1608653..1609144	-	492	WP_007052625.1	type II toxin-antitoxin system VapC family toxin	Toxin
GTQ89_RS06720	1609141..1609533	-	393	WP_007057212.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GTQ89_RS06725	1609678..1612377	-	2700	WP_115768916.1	aconitate hydratase AcnA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-146)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-61)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18531.70 Da
• Isoelectric Point: 6.2348

>T148644 WP_007052625.1 NZ_CP048001:c1609144-1608653 [Bifidobacterium longum]
MRQSKQVSILCDTNILIYYMLCTPRFIKDVMKLFERCVEREVKLSCASLSLKDVFYITPERFKERFMNDESMVVATTAKL
ILPEVAWSGISKLKELMTIVPIGAEECELAQSLRHAHNDFEDDLIIAAAQQSGATYVVTYDKKLAKHFPEYCKTPAEIVA
ALA

Nucleotide

• Length: 492 bp

>T148644 NZ_CP063814:1434637-1434897 [Microbacterium sp. A18JL200]
GTGACGCGATCGCTTGCCTTCGACCCGAACGGGTGGGAGGACTACGTCTACTGGCAGACACAGGACCGCAAGACGCTGAA
GCGGATCAACCTGCTGATCGCGGATGTCGTTCGTGATCCGTTCGACGGCATCGGGAAACCCGAACCGCTCCGTCACGTTC
TTTCCGGCGCCTGGTCTCGTCGCATCGACGAGAAGAACCGACTCGTCTATTACGTGACCGATGACCACATCGTCATACTG
CAGGCGCGAGACCACTACTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15147.29 Da
• Isoelectric Point: 5.8624

>AT148644 WP_007057212.1 NZ_CP048001:c1609533-1609141 [Bifidobacterium longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREAEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDEIADERLKEYRHQSDYARYLEKERSLS

Nucleotide

• Length: 393 bp

>AT148644 NZ_CP063814:1434389-1434640 [Microbacterium sp. A18JL200]
ATGTCCGCGATCACCGCGACCGAAGCGCGAAAGAGCCTGTTCGGGCTCATTCAGCAGGTCAACGAGGACCACACGACCGT
TGAAGTCGTCTCCCGCCGCGGCAACGCTGTGATCATGTCCAAGGACGACTTCGACGCACTGACCGAGACGGCCTACCTCC
TGCGCAACCCTGCGAACGCGGAGCGACTGCTCGAGGCTGTCGAGCGCGCACGCCGCGGCGAGTTTCAGCAGCACGACCTC
CTTGACGCGTGA

Structures

Toxin

• AlphaFold DB: F8APN2

Antitoxin

• AlphaFold DB: F8APN1