Detailed information of TA system TA148643

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-yefM/YoeB-RelB
• Location: 7722..8238
• Replicon: plasmid pVPTJ15-2
• Accession: NZ_CP047998
• Organism: Vibrio parahaemolyticus strain 20150710009

Toxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GSS20_RS24280
• Protein ID: WP_140220082.1
• Coordinates: 7969..8238 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: U2ZH00
• Locus tag: GSS20_RS24275
• Protein ID: WP_005526636.1
• Coordinates: 7722..7976 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSS20_RS24260 (GSS20_25570)	3164..3784	+	621	WP_140391890.1	recombinase family protein	-
GSS20_RS24265 (GSS20_25575)	3788..6784	+	2997	WP_140391851.1	Tn3 family transposase	-
GSS20_RS24270 (GSS20_25580)	6814..7389	-	576	Protein_6	recombinase family protein	-
GSS20_RS24275 (GSS20_25585)	7722..7976	+	255	WP_005526636.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GSS20_RS24280 (GSS20_25590)	7969..8238	+	270	WP_140220082.1	Txe/YoeB family addiction module toxin	Toxin
GSS20_RS24285 (GSS20_25595)	8756..9025	+	270	WP_140220080.1	transposase	-
GSS20_RS24290 (GSS20_25600)	9055..9738	-	684	WP_140220078.1	IS6 family transposase	-
GSS20_RS24295 (GSS20_25605)	9808..10626	+	819	Protein_11	IS3 family transposase	-
GSS20_RS24300 (GSS20_25610)	10789..11223	-	435	WP_140220074.1	hypothetical protein	-
GSS20_RS24305 (GSS20_25615)	11303..11515	-	213	WP_140220072.1	hypothetical protein	-
GSS20_RS24310 (GSS20_25620)	11671..11871	+	201	Protein_14	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..71782	71782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (10-89)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10618.95 Da
• Isoelectric Point: 7.9816

>T148643 WP_140220082.1 NZ_CP047998:7969-8238 [Vibrio parahaemolyticus]
MSSSQRLLSWTDDAWDDYLYWQTQDKKTLKRINKLINDIKRSSFEGIGKPEPLKENLSGFWSRRIDDTNRLVYAVDDQAI
TIISCRYHY

Nucleotide

• Length: 270 bp

>T148643 NZ_CP063814:1434389-1434640 [Microbacterium sp. A18JL200]
ATGTCCGCGATCACCGCGACCGAAGCGCGAAAGAGCCTGTTCGGGCTCATTCAGCAGGTCAACGAGGACCACACGACCGT
TGAAGTCGTCTCCCGCCGCGGCAACGCTGTGATCATGTCCAAGGACGACTTCGACGCACTGACCGAGACGGCCTACCTCC
TGCGCAACCCTGCGAACGCGGAGCGACTGCTCGAGGCTGTCGAGCGCGCACGCCGCGGCGAGTTTCAGCAGCACGACCTC
CTTGACGCGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9453.60 Da
• Isoelectric Point: 5.0623

>AT148643 WP_005526636.1 NZ_CP047998:7722-7976 [Vibrio parahaemolyticus]
MRIVSFTEARNGLKAVLDGVVNDADTTVITRRDSEDAVVMSLDYYNSLMETVHLLRSPQNAEHLNRSIAQYRAGKTTARE
LIDE

Nucleotide

• Length: 255 bp

>AT148643 NZ_CP063814:1434637-1434897 [Microbacterium sp. A18JL200]
GTGACGCGATCGCTTGCCTTCGACCCGAACGGGTGGGAGGACTACGTCTACTGGCAGACACAGGACCGCAAGACGCTGAA
GCGGATCAACCTGCTGATCGCGGATGTCGTTCGTGATCCGTTCGACGGCATCGGGAAACCCGAACCGCTCCGTCACGTTC
TTTCCGGCGCCTGGTCTCGTCGCATCGACGAGAAGAACCGACTCGTCTATTACGTGACCGATGACCACATCGTCATACTG
CAGGCGCGAGACCACTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A244CM06