Detailed information of TA system TA148637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 50602..51170
• Replicon: plasmid pHZ52-3
• Accession: NZ_CP047989
• Organism: Vibrio parahaemolyticus strain HZ-52

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1Y1BA95
• Locus tag: GSS61_RS25880
• Protein ID: WP_023624650.1
• Coordinates: 50829..51170 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GSS61_RS25875
• Protein ID: WP_017449238.1
• Coordinates: 50602..50832 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSS61_RS25850 (GSS61_27095)	45948..46979	-	1032	WP_159408187.1	Ig-like domain-containing protein	-
GSS61_RS25855 (GSS61_27100)	47540..47914	-	375	WP_127914005.1	hypothetical protein	-
GSS61_RS25860 (GSS61_27105)	48224..48445	+	222	WP_023624649.1	hypothetical protein	-
GSS61_RS25865 (GSS61_27110)	49048..49431	+	384	Protein_64	recombinase family protein	-
GSS61_RS25870 (GSS61_27115)	49426..50028	-	603	WP_224067207.1	site-specific integrase	-
GSS61_RS25875 (GSS61_27120)	50602..50832	+	231	WP_017449238.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GSS61_RS25880 (GSS61_27125)	50829..51170	+	342	WP_023624650.1	endoribonuclease MazF	Toxin
GSS61_RS25885 (GSS61_27130)	51473..52048	-	576	WP_017449240.1	hypothetical protein	-
GSS61_RS25890 (GSS61_27135)	52048..52779	-	732	WP_025789518.1	AHH domain-containing protein	-
GSS61_RS25895 (GSS61_27140)	53040..53131	-	92	Protein_70	DUF3265 domain-containing protein	-
GSS61_RS25900 (GSS61_27145)	54022..55110	-	1089	WP_086370877.1	replication initiation protein	-
GSS61_RS25905 (GSS61_27150)	55444..56082	-	639	Protein_72	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106397	106397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-107)

Antitoxin

• Antitoxin PemI-like (1-75)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12159.65 Da
• Isoelectric Point: 5.8124

>T148637 WP_023624650.1 NZ_CP047989:50829-51170 [Vibrio parahaemolyticus]
MTYAVPEKGDVVVMSFNPQSGHEQAGDSRPGLVISPKEFNQATGFALVCPITNQSKGYPFEVEITGSQKTTGVVLADQVK
SLDWRARRARVVDSVSNETLGTVRDYVTAIANG

Nucleotide

• Length: 342 bp

>T148637 NZ_CP063804:c3647186-3646758 [Mycobacterium orygis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8499.87 Da
• Isoelectric Point: 6.4604

>AT148637 WP_017449238.1 NZ_CP047989:50602-50832 [Vibrio parahaemolyticus]
MSQTIAKWGHSAAVRIPSKVLKNMDMAVGDKVEVKVFNRQIIIEPAKPTLAQLLSQITDENRHDEVITETMGNELL

Nucleotide

• Length: 231 bp

>AT148637 NZ_CP063804:c3647432-3647190 [Mycobacterium orygis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A1Y1BA95