Detailed information of TA system TA148636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2313781..2314544
• Replicon: chromosome
• Accession: NZ_CP047985
• Organism: Vibrio parahaemolyticus strain HZ-52

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A919BFZ0
• Locus tag: GSS61_RS10915
• Protein ID: WP_023584043.1
• Coordinates: 2314038..2314544 (+)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: GSS61_RS10910
• Protein ID: WP_000212004.1
• Coordinates: 2313781..2314047 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GSS61_RS10900 (GSS61_11405)	2309133..2310239	-	1107	WP_193239116.1	hypothetical protein	-
GSS61_RS10905 (GSS61_11410)	2310236..2312965	-	2730	WP_193239115.1	DEAD/DEAH box helicase	-
GSS61_RS10910 (GSS61_11415)	2313781..2314047	+	267	WP_000212004.1	DUF1778 domain-containing protein	Antitoxin
GSS61_RS10915 (GSS61_11420)	2314038..2314544	+	507	WP_023584043.1	GNAT family N-acetyltransferase	Toxin
GSS61_RS10920 (GSS61_11425)	2314585..2314971	-	387	WP_023584042.1	TraA family conjugative transfer protein	-
GSS61_RS10925 (GSS61_11430)	2314968..2315540	-	573	WP_001944091.1	type IV conjugative transfer system lipoprotein TraV	-
GSS61_RS10930 (GSS61_11435)	2315615..2316904	-	1290	WP_193239114.1	TraB/VirB10 family protein	-
GSS61_RS10935 (GSS61_11440)	2316907..2317803	-	897	WP_193234406.1	type-F conjugative transfer system secretin TraK	-
GSS61_RS10940 (GSS61_11445)	2317787..2318413	-	627	WP_000667170.1	TraE/TraK family type IV conjugative transfer system protein	-
GSS61_RS10945 (GSS61_11450)	2318410..2318691	-	282	WP_000433889.1	type IV conjugative transfer system protein TraL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2290042..2346694	56652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-83)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 18278.00 Da
• Isoelectric Point: 7.2132

>T148636 WP_023584043.1 NZ_CP047985:2314038-2314544 [Vibrio parahaemolyticus]
MGISAPTLLTDEHQLNAFQCGIEELDTWLRKQALKSQKRGTARVYVVNDTDAHQVVGYYAIAMGSVSREQAFSSLRRNSP
DPIPMVILARLGVDNAYQGQGIAAGLLKDCIIRSVQAMNAVGGAGILVHAIDGSAQTFYKKFGFKESTFDPLVLMARICD
IEKSLSID

Nucleotide

• Length: 507 bp

>T148636 NZ_CP063804:3129636-3130079 [Mycobacterium orygis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9956.48 Da
• Isoelectric Point: 5.8580

>AT148636 WP_000212004.1 NZ_CP047985:2313781-2314047 [Vibrio parahaemolyticus]
MATKTAPINMRVLPSVRDIIDAAASLKKVDRTVFIQQAALNEAHSILAEQRDFVLEAKAFEAFDNELRAEPQTLEGMKDL
FKRKAPWE

Nucleotide

• Length: 267 bp

>AT148636 NZ_CP063804:3129392-3129649 [Mycobacterium orygis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA