Detailed information of TA system TA148623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 64101..64689
• Replicon: plasmid pDETAB2
• Accession: NZ_CP047975
• Organism: Acinetobacter baumannii strain DETAB-P2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N8SM64
• Locus tag: GWK28_RS19415
• Protein ID: WP_000438825.1
• Coordinates: 64402..64689 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: D3JXQ4
• Locus tag: GWK28_RS19410
• Protein ID: WP_004728120.1
• Coordinates: 64101..64415 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK28_RS19380	59327..60358	+	1032	WP_004201169.1	protein-disulfide reductase DsbD N-terminal domain-containing protein	-
GWK28_RS19385	60369..61007	-	639	WP_004201168.1	phosphoribosylanthranilate isomerase	-
GWK28_RS19390	61012..61377	-	366	WP_004201167.1	bleomycin binding protein Ble-MBL	-
GWK28_RS19395	61381..62193	-	813	WP_004201164.1	subclass B1 metallo-beta-lactamase NDM-1	-
GWK28_RS19400	62294..63319	-	1026	WP_001988464.1	IS30-like element ISAba125 family transposase	-
GWK28_RS19405	63482..64012	+	531	WP_000172759.1	hypothetical protein	-
GWK28_RS19410	64101..64415	-	315	WP_004728120.1	BrnA antitoxin family protein	Antitoxin
GWK28_RS19415	64402..64689	-	288	WP_000438825.1	BrnT family toxin	Toxin
GWK28_RS19420	64933..65703	-	771	WP_032073134.1	hypothetical protein	-
GWK28_RS19425	65762..66100	+	339	WP_032073153.1	hypothetical protein	-
GWK28_RS19430	66228..66800	-	573	WP_004992249.1	IS6 family transposase	-
GWK28_RS19435	66864..67694	+	831	Protein_69	IS91-like element ISVsa3 family transposase	-
GWK28_RS19445	67930..68172	-	243	Protein_70	phosphoglucosamine mutase	-
GWK28_RS19450	68259..69074	-	816	WP_001043260.1	sulfonamide-resistant dihydropteroate synthase Sul2	-
GWK28_RS19455	69164..69511	+	348	Protein_72	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / msr(E) / mph(E) / tet(39)	-	1..100072	100072
	-	inside	IScluster/Tn	blaNDM-1 / sul2	-	61510..70294	8784

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11304.70 Da
• Isoelectric Point: 5.6205

>T148623 WP_000438825.1 NZ_CP047975:c64689-64402 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T148623 NZ_CP063804:c2378597-2378280 [Mycobacterium orygis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12200.86 Da
• Isoelectric Point: 8.4628

>AT148623 WP_004728120.1 NZ_CP047975:c64415-64101 [Acinetobacter baumannii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT148623 NZ_CP063804:c2378809-2378594 [Mycobacterium orygis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A0A8TVQ4

Antitoxin

• AlphaFold DB: A0A0A8TRI9