Detailed information of TA system TA148622

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 52647..53231
• Replicon: plasmid pDETAB2
• Accession: NZ_CP047975
• Organism: Acinetobacter baumannii strain DETAB-P2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N8Q7V3
• Locus tag: GWK28_RS19345
• Protein ID: WP_000286964.1
• Coordinates: 52929..53231 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GWK28_RS19340
• Protein ID: WP_000985609.1
• Coordinates: 52647..52928 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK28_RS19315	49289..49735	-	447	Protein_45	AraC family ligand binding domain-containing protein	-
GWK28_RS19320	49863..50615	-	753	Protein_46	IS1-like element ISAba3 family transposase	-
GWK28_RS19325	50706..51548	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
GWK28_RS19330	51629..51997	+	369	Protein_48	IS1-like element ISAba3 family transposase	-
GWK28_RS19335	52148..52567	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
GWK28_RS19340	52647..52928	-	282	WP_000985609.1	putative addiction module antidote protein	Antitoxin
GWK28_RS19345	52929..53231	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GWK28_RS19350	53282..54307	-	1026	WP_001988464.1	IS30-like element ISAba125 family transposase	-
GWK28_RS19355	54600..54884	+	285	WP_015056391.1	hypothetical protein	-
GWK28_RS19360	55095..56624	-	1530	WP_015056392.1	IS91 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / msr(E) / mph(E) / tet(39)	-	1..100072	100072
	-	inside	IScluster/Tn	blaOXA-58	-	50178..54307	4129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-90)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11573.07 Da
• Isoelectric Point: 4.6838

>T148622 WP_000286964.1 NZ_CP047975:c53231-52929 [Acinetobacter baumannii]
MYSIYTTEVFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T148622 NZ_CP063804:c2341907-2341473 [Mycobacterium orygis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT148622 WP_000985609.1 NZ_CP047975:c52928-52647 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEDDMQAYLQACIEESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT148622 NZ_CP063804:c2342168-2341914 [Mycobacterium orygis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Toxin

• AlphaFold DB: A0A0A8TV94