Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 199924..200577 Replicon chromosome
Accession NZ_CP047971
Organism Kyrpidia spormannii strain FAVT5

Toxin (Protein)


Gene name mazF Uniprot ID D5WSA7
Locus tag GWK40_RS01120 Protein ID WP_013074285.1
Coordinates 200227..200577 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2K8N2G8
Locus tag GWK40_RS01115 Protein ID WP_041303528.1
Coordinates 199924..200223 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK40_RS16310 195386..195718 + 333 WP_252346453.1 hypothetical protein -
GWK40_RS01100 195735..197246 + 1512 WP_170098987.1 NAD(P)H-hydrate dehydratase -
GWK40_RS01105 197327..198319 + 993 WP_170084685.1 outer membrane lipoprotein carrier protein LolA -
GWK40_RS01110 198330..199421 - 1092 WP_170084686.1 cation diffusion facilitator family transporter -
GWK40_RS01115 199924..200223 + 300 WP_041303528.1 CopG family ribbon-helix-helix protein Antitoxin
GWK40_RS01120 200227..200577 + 351 WP_013074285.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GWK40_RS01125 200636..201895 + 1260 WP_170098988.1 adenosylhomocysteinase -
GWK40_RS01130 202051..202791 + 741 WP_170084688.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
GWK40_RS01135 203083..203304 + 222 WP_013074288.1 DUF1659 domain-containing protein -
GWK40_RS01140 203340..203573 + 234 WP_170084689.1 DUF2922 domain-containing protein -
GWK40_RS01145 203689..204282 - 594 WP_100666597.1 DedA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-53)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12906.94 Da        Isoelectric Point: 5.6976

>T148615 WP_013074285.1 NZ_CP047971:200227-200577 [Kyrpidia spormannii]
VNIKRGDIFFANLSPVVGSEQGGFRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGLDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMSKVNESLMISLGLIDF

Download         Length: 351 bp

>T148615 NZ_CP063804:2188850-2189227 [Mycobacterium orygis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 11329.99 Da        Isoelectric Point: 4.9577

>AT148615 WP_041303528.1 NZ_CP047971:199924-200223 [Kyrpidia spormannii]
MASVPEVLGLSETKRIMISVPSHLLQEVDGIAERENSNRSEVIRQAMRLYLEERKKERIRELMQRGYVEMAKINLYIASE
AFYAEEEAGSTLDRLVSGV

Download         Length: 300 bp

>AT148615 NZ_CP063804:2189269-2189718 [Mycobacterium orygis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K8N5K4

Antitoxin

Source ID Structure
AlphaFold DB A0A2K8N2G8

References