Detailed information of TA system TA148613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 710557..711470
• Replicon: chromosome
• Accession: NZ_CP047970
• Organism: Cobetia pacifica strain GPM2

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FX986_RS02800
• Protein ID: WP_191237822.1
• Coordinates: 711012..711470 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A4Y4GLB4
• Locus tag: FX986_RS02795
• Protein ID: WP_024952500.1
• Coordinates: 710557..711009 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FX986_RS02770	707537..708190	-	654	WP_191237363.1	SWF/SNF helicase family protein	-
FX986_RS02775	708386..708664	+	279	WP_191237364.1	hypothetical protein	-
FX986_RS02780	708817..709281	+	465	WP_191237365.1	methylenetetrahydrofolate reductase C-terminal domain-containing protein	-
FX986_RS02785	709278..710282	+	1005	WP_191237366.1	methylenetetrahydrofolate reductase	-
FX986_RS02790	710336..710524	+	189	WP_191237367.1	hypothetical protein	-
FX986_RS02795	710557..711009	+	453	WP_024952500.1	DUF2384 domain-containing protein	Antitoxin
FX986_RS02800	711012..711470	+	459	WP_191237822.1	RES family NAD+ phosphorylase	Toxin
FX986_RS02805	712276..712620	-	345	Protein_559	IS30 family transposase	-
FX986_RS02810	712906..713091	+	186	WP_141392016.1	DUF2188 domain-containing protein	-
FX986_RS02815	714296..715021	+	726	WP_191237368.1	gluconate 2-dehydrogenase subunit 3 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	702305..724921	22616

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (5-147)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (96-147)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17083.49 Da
• Isoelectric Point: 4.5316

>T148613 WP_191237822.1 NZ_CP047970:711012-711470 [Cobetia pacifica]
VVRGYRLVKRKWLKTAFDGEGARLYGGRWNSKGKACVYLASTESLAMLEVMVHLEDNQLLKEYALLEVTFREEVLMQLPE
DVLPADWRAEPAPPSTAEIGDDWLEGQSSLVLAVPSVVAPRETNYLVNPEHPAFLALAQSAEEVEFTPDRRL

Nucleotide

• Length: 459 bp

>T148613 NZ_CP063804:1972693-1972941 [Mycobacterium orygis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATACGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16538.10 Da
• Isoelectric Point: 10.1343

>AT148613 WP_024952500.1 NZ_CP047970:710557-711009 [Cobetia pacifica]
MQAAIKEYRPKPAARKDDFWRALGLPSRGAKLHEALSAGISYKVYTKLTEASGLESKELARYVVIPPATLKRRADTGYFK
PAEGDRLYRFAEVYKSAVDLFEGDKAKAKEWLLSPVRGLGGRRPVEMVATTAGAEAVLDLIGRLEHGVFA

Nucleotide

• Length: 453 bp

>AT148613 NZ_CP063804:1972481-1972693 [Mycobacterium orygis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A4Y4GLB4