Detailed information of TA system TA148612

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 80296..81093
• Replicon: plasmid pECL189-2
• Accession: NZ_CP047967
• Organism: Enterobacter hormaechei strain 189

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GUY47_RS25455
• Protein ID: WP_172754447.1
• Coordinates: 80296..80799 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: GUY47_RS25460
• Protein ID: WP_016582626.1
• Coordinates: 80824..81093 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY47_RS25425	76885..77901	+	1017	Protein_87	tail fiber domain-containing protein	-
GUY47_RS25430	77966..78754	+	789	WP_040110304.1	receptor-recognizing protein	-
GUY47_RS25435	78829..79152	+	324	WP_000064173.1	hypothetical protein	-
GUY47_RS25440	79166..79858	+	693	WP_006812500.1	membrane protein	-
GUY47_RS25445	79860..80111	+	252	WP_040110303.1	hypothetical protein	-
GUY47_RS25450	80062..80268	+	207	WP_080332520.1	hypothetical protein	-
GUY47_RS25455	80296..80799	-	504	WP_172754447.1	GNAT family N-acetyltransferase	Toxin
GUY47_RS25460	80824..81093	-	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
GUY47_RS25465	81510..82175	+	666	WP_023315962.1	AAA family ATPase	-
GUY47_RS25470	82181..82534	+	354	WP_160956455.1	hypothetical protein	-
GUY47_RS25475	82576..83373	-	798	WP_077990606.1	DUF2971 domain-containing protein	-
GUY47_RS25480	83637..84362	+	726	WP_006812583.1	hypothetical protein	-
GUY47_RS25485	84423..85763	+	1341	WP_006812582.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..110177	110177

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18603.30 Da
• Isoelectric Point: 8.8869

>T148612 WP_172754447.1 NZ_CP047967:c80799-80296 [Enterobacter hormaechei]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T148612 NZ_CP063804:c1952138-1951749 [Mycobacterium orygis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT148612 WP_016582626.1 NZ_CP047967:c81093-80824 [Enterobacter hormaechei]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT148612 NZ_CP063804:c1952362-1952135 [Mycobacterium orygis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3