Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32368..33011 Replicon plasmid pECL189-1
Accession NZ_CP047966
Organism Enterobacter hormaechei strain 189

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag GUY47_RS24380 Protein ID WP_001044770.1
Coordinates 32368..32784 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag GUY47_RS24385 Protein ID WP_001261282.1
Coordinates 32781..33011 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUY47_RS24345 27910..28146 - 237 WP_000993386.1 broad-spectrum mercury transporter MerE -
GUY47_RS24350 28143..28505 - 363 WP_001277456.1 mercury resistance co-regulator MerD -
GUY47_RS24355 28523..30217 - 1695 WP_000105636.1 mercury(II) reductase -
GUY47_RS24360 30269..30691 - 423 WP_001340589.1 organomercurial transporter MerC -
GUY47_RS24365 30727..30852 - 126 WP_072093211.1 mercury transporter -
GUY47_RS24370 30853..31550 - 698 Protein_36 IS1 family transposase -
GUY47_RS24375 31584..32294 - 711 WP_004152334.1 ATP-binding protein -
GUY47_RS24380 32368..32784 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
GUY47_RS24385 32781..33011 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GUY47_RS24390 32968..33195 + 228 Protein_40 hypothetical protein -
GUY47_RS24395 33522..33974 - 453 WP_004201034.1 hypothetical protein -
GUY47_RS24400 34393..34638 + 246 Protein_42 hypothetical protein -
GUY47_RS24405 34782..35132 + 351 WP_000493378.1 hypothetical protein -
GUY47_RS24410 35183..35926 + 744 WP_000129823.1 hypothetical protein -
GUY47_RS24415 35923..36699 + 777 WP_000015958.1 site-specific integrase -
GUY47_RS24420 36757..37014 - 258 WP_000764642.1 hypothetical protein -
GUY47_RS24425 37143..37247 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / catA2 / tet(D) / dfrA14 / blaSHV-12 / blaKPC-2 - 1..133022 133022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T148611 WP_001044770.1 NZ_CP047966:c32784-32368 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T148611 NZ_CP063804:1776374-1776778 [Mycobacterium orygis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT148611 WP_001261282.1 NZ_CP047966:c33011-32781 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT148611 NZ_CP063804:1776165-1776368 [Mycobacterium orygis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References