Detailed information of TA system TA148610

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4938720..4939336
• Replicon: chromosome
• Accession: NZ_CP047965
• Organism: Enterobacter hormaechei strain 189

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GUY47_RS23780
• Protein ID: WP_077266491.1
• Coordinates: 4938720..4939091 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: GUY47_RS23785
• Protein ID: WP_015569912.1
• Coordinates: 4939094..4939336 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY47_RS23765	4936220..4937122	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
GUY47_RS23770	4937119..4937754	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
GUY47_RS23775	4937751..4938680	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
GUY47_RS23780	4938720..4939091	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
GUY47_RS23785	4939094..4939336	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
GUY47_RS23790	4939535..4940455	+	921	WP_032652004.1	alpha/beta hydrolase	-
GUY47_RS23795	4940464..4941405	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
GUY47_RS23800	4941450..4941887	-	438	WP_015569909.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GUY47_RS23805	4941884..4942765	-	882	WP_003861949.1	virulence factor BrkB family protein	-
GUY47_RS23810	4942759..4943358	-	600	WP_003861947.1	glucose-1-phosphatase	-
GUY47_RS23815	4943477..4944277	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T148610 WP_077266491.1 NZ_CP047965:c4939091-4938720 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T148610 NZ_CP063804:1695810-1696127 [Mycobacterium orygis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT148610 WP_015569912.1 NZ_CP047965:c4939336-4939094 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT148610 NZ_CP063804:1695511-1695813 [Mycobacterium orygis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGTGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3