Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 1096319..1096998 Replicon chromosome
Accession NZ_CP047965
Organism Enterobacter hormaechei strain 189

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag GUY47_RS05230 Protein ID WP_000854680.1
Coordinates 1096319..1096660 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A5C1BXL1
Locus tag GUY47_RS05235 Protein ID WP_023336793.1
Coordinates 1096681..1096998 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUY47_RS05205 1091490..1091891 + 402 WP_006809999.1 sigma factor-binding protein Crl -
GUY47_RS05210 1092024..1093076 - 1053 WP_017383128.1 phosphoporin PhoE -
GUY47_RS05215 1093382..1094485 + 1104 WP_032652660.1 glutamate 5-kinase -
GUY47_RS05220 1094497..1095750 + 1254 WP_032652662.1 glutamate-5-semialdehyde dehydrogenase -
GUY47_RS05230 1096319..1096660 - 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
GUY47_RS05235 1096681..1096998 - 318 WP_023336793.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
GUY47_RS05240 1097017..1097238 - 222 WP_000691994.1 DUF987 domain-containing protein -
GUY47_RS05245 1097247..1097723 - 477 WP_023336794.1 RadC family protein -
GUY47_RS05250 1097739..1098197 - 459 WP_000211838.1 antirestriction protein -
GUY47_RS05255 1098295..1098534 - 240 WP_000194654.1 DUF905 domain-containing protein -
GUY47_RS05260 1098611..1099078 - 468 WP_001581459.1 hypothetical protein -
GUY47_RS05265 1099102..1099545 - 444 WP_000824223.1 hypothetical protein -
GUY47_RS05270 1099545..1099781 - 237 WP_001144031.1 hypothetical protein -
GUY47_RS05275 1099822..1100523 - 702 WP_006812588.1 WYL domain-containing protein -
GUY47_RS05280 1100740..1101561 - 822 WP_063613361.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T148594 WP_000854680.1 NZ_CP047965:c1096660-1096319 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T148594 NZ_CP063804:705641-706042 [Mycobacterium orygis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12016.63 Da        Isoelectric Point: 7.0270

>AT148594 WP_023336793.1 NZ_CP047965:c1096998-1096681 [Enterobacter hormaechei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT148594 NZ_CP063804:705399-705644 [Mycobacterium orygis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BXL1

References