Detailed information of TA system TA148594

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1096319..1096998
• Replicon: chromosome
• Accession: NZ_CP047965
• Organism: Enterobacter hormaechei strain 189

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: GUY47_RS05230
• Protein ID: WP_000854680.1
• Coordinates: 1096319..1096660 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A5C1BXL1
• Locus tag: GUY47_RS05235
• Protein ID: WP_023336793.1
• Coordinates: 1096681..1096998 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY47_RS05205	1091490..1091891	+	402	WP_006809999.1	sigma factor-binding protein Crl	-
GUY47_RS05210	1092024..1093076	-	1053	WP_017383128.1	phosphoporin PhoE	-
GUY47_RS05215	1093382..1094485	+	1104	WP_032652660.1	glutamate 5-kinase	-
GUY47_RS05220	1094497..1095750	+	1254	WP_032652662.1	glutamate-5-semialdehyde dehydrogenase	-
GUY47_RS05230	1096319..1096660	-	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
GUY47_RS05235	1096681..1096998	-	318	WP_023336793.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
GUY47_RS05240	1097017..1097238	-	222	WP_000691994.1	DUF987 domain-containing protein	-
GUY47_RS05245	1097247..1097723	-	477	WP_023336794.1	RadC family protein	-
GUY47_RS05250	1097739..1098197	-	459	WP_000211838.1	antirestriction protein	-
GUY47_RS05255	1098295..1098534	-	240	WP_000194654.1	DUF905 domain-containing protein	-
GUY47_RS05260	1098611..1099078	-	468	WP_001581459.1	hypothetical protein	-
GUY47_RS05265	1099102..1099545	-	444	WP_000824223.1	hypothetical protein	-
GUY47_RS05270	1099545..1099781	-	237	WP_001144031.1	hypothetical protein	-
GUY47_RS05275	1099822..1100523	-	702	WP_006812588.1	WYL domain-containing protein	-
GUY47_RS05280	1100740..1101561	-	822	WP_063613361.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T148594 WP_000854680.1 NZ_CP047965:c1096660-1096319 [Enterobacter hormaechei]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T148594 NZ_CP063804:705641-706042 [Mycobacterium orygis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12016.63 Da
• Isoelectric Point: 7.0270

>AT148594 WP_023336793.1 NZ_CP047965:c1096998-1096681 [Enterobacter hormaechei]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT148594 NZ_CP063804:705399-705644 [Mycobacterium orygis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: A0A5C1BXL1