Detailed information of TA system TA148591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 13012..13531
• Replicon: plasmid pMC64B
• Accession: NZ_CP047964
• Organism: Aeromonas media strain MC64

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GWI30_RS23200
• Protein ID: WP_113722067.1
• Coordinates: 13012..13299 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S1GNF0
• Locus tag: GWI30_RS23205
• Protein ID: WP_026080514.1
• Coordinates: 13289..13531 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWI30_RS23165	8701..8943	+	243	WP_103859311.1	plasmid replication protein RepB	-
GWI30_RS23170	9781..9978	-	198	WP_161508007.1	chromosome partitioning protein ParB	-
GWI30_RS23175	9999..10628	-	630	WP_042044880.1	AAA family ATPase	-
GWI30_RS23180	10916..10999	-	84	WP_161508013.1	AAA family ATPase	-
GWI30_RS23185	11204..11491	+	288	WP_101617265.1	CopG family ribbon-helix-helix protein	-
GWI30_RS23190	11479..11772	+	294	WP_101617266.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GWI30_RS23195	12621..12962	-	342	WP_161508008.1	type II toxin-antitoxin system CcdA family antitoxin	-
GWI30_RS23200	13012..13299	-	288	WP_113722067.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GWI30_RS23205	13289..13531	-	243	WP_026080514.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GWI30_RS23210	13962..15548	+	1587	WP_161508009.1	mobilization protein	-
GWI30_RS23215	15553..15798	-	246	WP_104456137.1	plasmid replication protein RepB	-
GWI30_RS23220	16664..17350	-	687	WP_139728813.1	NYN domain-containing protein	-
GWI30_RS23225	17576..17845	-	270	WP_039062313.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..24044	24044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (2-62)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11280.08 Da
• Isoelectric Point: 9.9994

>T148591 WP_113722067.1 NZ_CP047964:c13299-13012 [Aeromonas media]
MTFELEFDPRAWKEWKKLGDTVRQQFKKKLAEVLERPRVEANRLHGLPDCYKIKLRGAGYRLVYQVQDDRVLVFVVAVGK
REREQVYLDAGSRLE

Nucleotide

• Length: 288 bp

>T148591 NZ_CP063804:680073-680480 [Mycobacterium orygis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9057.37 Da
• Isoelectric Point: 4.6017

>AT148591 WP_026080514.1 NZ_CP047964:c13531-13289 [Aeromonas media]
MGTINLRIDDDLKTRSYAVLEKLGVTPSELLRQTLEYVASQERLPFRTTVVTEDEEALLALVRERLASPQKGIRVSLDDL

Nucleotide

• Length: 243 bp

>AT148591 NZ_CP063804:679861-680076 [Mycobacterium orygis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S1GNF0