Detailed information of TA system TA148569

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1578843..1579624
• Replicon: chromosome
• Accession: NZ_CP047943
• Organism: Synechococcus sp. RS9909

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: SynRS9909_RS08075
• Protein ID: WP_038001288.1
• Coordinates: 1579115..1579624 (+)
• Length: 170 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A3Z7D8
• Locus tag: SynRS9909_RS08070
• Protein ID: WP_007102268.1
• Coordinates: 1578843..1579115 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SynRS9909_RS08055	1575180..1577045	-	1866	WP_007102271.1	DUF2075 domain-containing protein	-
SynRS9909_RS08060	1577176..1578090	-	915	WP_116431415.1	HIT domain-containing protein	-
SynRS9909_RS08065	1578476..1578733	+	258	WP_007102269.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
SynRS9909_RS08070	1578843..1579115	+	273	WP_007102268.1	DUF1778 domain-containing protein	Antitoxin
SynRS9909_RS08075	1579115..1579624	+	510	WP_038001288.1	GNAT family N-acetyltransferase	Toxin
SynRS9909_RS08080	1579891..1580277	-	387	WP_038001960.1	hypothetical protein	-
SynRS9909_RS08085	1580368..1580811	+	444	WP_007102264.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
SynRS9909_RS08090	1580831..1582105	+	1275	WP_007102263.1	Y-family DNA polymerase	-
SynRS9909_RS08095	1582143..1583267	+	1125	WP_007102262.1	choloylglycine hydrolase family protein	-
SynRS9909_RS08100	1583275..1583571	-	297	WP_007102261.1	hypothetical protein	-
SynRS9909_RS08105	1583576..1583833	-	258	WP_007102260.1	hypothetical protein	-
SynRS9909_RS08110	1583934..1584083	-	150	Protein_1585	arsenical-resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (78-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-84)

Sequences

Toxin

Protein

• Length: 170 a.a.
• Molecular weight: 17804.48 Da
• Isoelectric Point: 8.3130

>T148569 WP_038001288.1 NZ_CP047943:1579115-1579624 [Synechococcus sp. RS9909]
MGLQAPQPLDDHHVMKGFDCGDHTLNQWLQQRALANQRSGATRTVVVCDADHAVKAYVALASGAVAVAASPGSFRRNMPD
PIPVVVLARLAVCRSVQGQGIARALLADAFERVLLTSQQIGVRGIVVHAAAAAARTFYLHMGFDSSPSDPSLLLLRLSDV
AAALEASAK

Nucleotide

• Length: 510 bp

>T148569 NZ_CP063775:49797-50177 [Escherichia coli O25b:H4-ST131]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10060.41 Da
• Isoelectric Point: 11.5657

>AT148569 WP_007102268.1 NZ_CP047943:1578843-1579115 [Synechococcus sp. RS9909]
MAATPRLSWNLRVSPGDQDLIDRAVRASGLTRTDFVLQAARAAAQNLLVEQTWSVLEPQAFETFRRQLDAPAGPNERLQR
TMAASRPWKA

Nucleotide

• Length: 273 bp

>AT148569 NZ_CP063775:49576-49797 [Escherichia coli O25b:H4-ST131]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A3Z7D8