Detailed information of TA system TA148565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 249821..250509
• Replicon: chromosome
• Accession: NZ_CP047943
• Organism: Synechococcus sp. RS9909

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A3Z4J2
• Locus tag: SynRS9909_RS01250
• Protein ID: WP_007100893.1
• Coordinates: 250060..250509 (+)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A3Z4J1
• Locus tag: SynRS9909_RS01245
• Protein ID: WP_007100894.1
• Coordinates: 249821..250060 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SynRS9909_RS01225	245911..247023	-	1113	WP_007100898.1	dTDP-glucose 4,6-dehydratase	-
SynRS9909_RS01230	247028..247933	-	906	WP_038001717.1	dTDP-4-dehydrorhamnose reductase	-
SynRS9909_RS01235	247945..248535	-	591	WP_007100896.1	dTDP-4-dehydrorhamnose 3,5-epimerase	-
SynRS9909_RS01240	248525..249565	-	1041	WP_007100895.1	glucose-1-phosphate thymidylyltransferase RfbA	-
SynRS9909_RS01245	249821..250060	+	240	WP_007100894.1	ribbon-helix-helix domain-containing protein	Antitoxin
SynRS9909_RS01250	250060..250509	+	450	WP_007100893.1	PIN domain-containing protein	Toxin
SynRS9909_RS01255	250665..251003	-	339	WP_007100892.1	type II toxin-antitoxin system PemK/MazF family toxin	-
SynRS9909_RS01260	250997..251428	-	432	WP_083774444.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
SynRS9909_RS01265	251618..251863	+	246	WP_071933942.1	type II toxin-antitoxin system ParD family antitoxin	-
SynRS9909_RS01270	251860..252228	+	369	WP_007100888.1	hypothetical protein	-
SynRS9909_RS01275	252335..252769	-	435	WP_007100887.1	PIN domain-containing protein	-
SynRS9909_RS01280	253117..254040	+	924	WP_007100885.1	N-acetylmuramoyl-L-alanine amidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16104.47 Da
• Isoelectric Point: 5.6894

>T148565 WP_007100893.1 NZ_CP047943:250060-250509 [Synechococcus sp. RS9909]
MDAPIALLDVNVLIALLDPQHVHHEPAHRWFQANASYGWATCPLTQNALLRILSNPRYPNSPGGPASVMPLLQGMLSHPG
HLFWPDLLSWSTDGELQAELLLHHGQITDTYLLALAVHQGGCLMSFDARLSTCAVPGGGDALCLIDSQV

Nucleotide

• Length: 450 bp

>T148565 NZ_CP063774:4132551-4132853 [Escherichia coli O25b:H4-ST131]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8709.89 Da
• Isoelectric Point: 4.7672

>AT148565 WP_007100894.1 NZ_CP047943:249821-250060 [Synechococcus sp. RS9909]
MRTTLQLDDDVLDAARVLARQQHLSVGEVISELARQALRRPAGLEEPPSERSGLPLLPIKSSGGVVDLNLVNQLRDEER

Nucleotide

• Length: 240 bp

>AT148565 NZ_CP063774:4132889-4133230 [Escherichia coli O25b:H4-ST131]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Toxin

• AlphaFold DB: A3Z4J2

Antitoxin

• AlphaFold DB: A3Z4J1