Detailed information of TA system TA148564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 93165..93829
• Replicon: chromosome
• Accession: NZ_CP047943
• Organism: Synechococcus sp. RS9909

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A3Z442
• Locus tag: SynRS9909_RS00490
• Protein ID: WP_007101039.1
• Coordinates: 93437..93829 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: SynRS9909_RS00485
• Protein ID: WP_116431295.1
• Coordinates: 93165..93434 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SynRS9909_RS00470	88547..89002	-	456	WP_007101044.1	GNAT family N-acetyltransferase	-
SynRS9909_RS00475	89093..91960	+	2868	WP_007101043.1	preprotein translocase subunit SecA	-
SynRS9909_RS00480	92284..93039	-	756	WP_007101041.1	serine O-acetyltransferase	-
SynRS9909_RS00485	93165..93434	+	270	WP_116431295.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
SynRS9909_RS00490	93437..93829	+	393	WP_007101039.1	type II toxin-antitoxin system VapC family toxin	Toxin
SynRS9909_RS00495	93815..94804	-	990	WP_007101038.1	GntR family transcriptional regulator	-
SynRS9909_RS00500	94880..95542	-	663	WP_007101037.1	translation initiation factor IF-3	-
SynRS9909_RS00505	95607..96527	-	921	WP_050752463.1	tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA	-
SynRS9909_RS00510	96646..98613	+	1968	WP_186593770.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-122)

Antitoxin

• Antitoxin VapB-like (1-88)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14169.31 Da
• Isoelectric Point: 5.1614

>T148564 WP_007101039.1 NZ_CP047943:93437-93829 [Synechococcus sp. RS9909]
MVIDTSALMAILKAEPEAADFLRCLSRSELSLLSTATRLETSLVVEGQLGAAGLPELELLLSRAGVETVPFELTHWHWAL
QGWRRYGKGRHRAALNLGDCFSYGLARALDQPLLFKGEDFALTDLASAGL

Nucleotide

• Length: 393 bp

>T148564 NZ_CP063774:c4103597-4103379 [Escherichia coli O25b:H4-ST131]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9823.13 Da
• Isoelectric Point: 6.2180

>AT148564 WP_116431295.1 NZ_CP047943:93165-93434 [Synechococcus sp. RS9909]
MSMNIKDPAVHAMARELAAHKGTTVTDAVRQALKAELARVGSASTSQAAEQREQQLMELLPRFRHLPWPEGVSSRELQDA
LYDDNGLPL

Nucleotide

• Length: 270 bp

>AT148564 NZ_CP063774:c4103997-4103623 [Escherichia coli O25b:H4-ST131]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A3Z442