Detailed information of TA system TA148563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 70821..71500
• Replicon: chromosome
• Accession: NZ_CP047943
• Organism: Synechococcus sp. RS9909

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A3Z418
• Locus tag: SynRS9909_RS00370
• Protein ID: WP_007101063.1
• Coordinates: 70821..71252 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A3Z419
• Locus tag: SynRS9909_RS00375
• Protein ID: WP_007101062.1
• Coordinates: 71252..71500 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SynRS9909_RS00350	66245..68254	+	2010	WP_007101067.1	ribonuclease J	-
SynRS9909_RS00355	68283..68960	-	678	WP_186593855.1	hypothetical protein	-
SynRS9909_RS00360	68917..69969	-	1053	WP_007101065.1	tRNA lysidine(34) synthetase TilS	-
SynRS9909_RS00365	70020..70793	+	774	WP_007101064.1	DUF561 domain-containing protein	-
SynRS9909_RS00370	70821..71252	-	432	WP_007101063.1	twitching motility protein PilT	Toxin
SynRS9909_RS00375	71252..71500	-	249	WP_007101062.1	hypothetical protein	Antitoxin
SynRS9909_RS00380	71612..71998	+	387	WP_007101061.1	phage holin family protein	-
SynRS9909_RS00385	72021..74756	-	2736	WP_007101060.1	DNA mismatch repair protein MutS	-
SynRS9909_RS00390	74809..75225	-	417	WP_007101059.1	PIN domain-containing protein	-
SynRS9909_RS00395	75225..75455	-	231	WP_007101058.1	DUF2191 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-125)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15542.86 Da
• Isoelectric Point: 7.8476

>T148563 WP_007101063.1 NZ_CP047943:c71252-70821 [Synechococcus sp. RS9909]
MRALLDINVIIALLDGQHSLHSTASQWLSHHAAAGWASCPISQNGVIRIMSQPSYPNAQPPAAIASRLAEACRHPSHQFW
GGALSLLDPRHLKWHCLLGPRQITDAYLLAVAVANDGCLVSFDRRIHHDLVPGATADQIVVVR

Nucleotide

• Length: 432 bp

>T148563 NZ_CP063774:3919770-3920690 [Escherichia coli O25b:H4-ST131]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.00 Da
• Isoelectric Point: 6.7133

>AT148563 WP_007101062.1 NZ_CP047943:c71500-71252 [Synechococcus sp. RS9909]
MRTTLDIDDDLLQAAKDLAKQQRMSAGQVVSTLLRQALTQAHHSPSEAAARPHPCGFRPFAGDPSRITNNADVEALRDAL
NL

Nucleotide

• Length: 249 bp

>AT148563 NZ_CP063774:3919001-3919783 [Escherichia coli O25b:H4-ST131]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A3Z418

Antitoxin

• AlphaFold DB: A3Z419