Detailed information of TA system TA148559

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/AbrB(antitoxin)
• Location: 1949708..1950292
• Replicon: chromosome
• Accession: NZ_CP047940
• Organism: Cyanobium sp. NS01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CyaNS01_RS10130
• Protein ID: WP_186696977.1
• Coordinates: 1949708..1950049 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CyaNS01_RS10135
• Protein ID: WP_186696978.1
• Coordinates: 1950074..1950292 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CyaNS01_RS10085	1945430..1945961	+	532	Protein_1984	hypothetical protein	-
CyaNS01_RS10090	1946171..1946326	-	156	WP_186696970.1	hypothetical protein	-
CyaNS01_RS10095	1946313..1946546	+	234	WP_186696971.1	DUF5320 domain-containing protein	-
CyaNS01_RS10100	1946575..1947150	-	576	WP_186696972.1	hypothetical protein	-
CyaNS01_RS10105	1947229..1947555	-	327	WP_186696973.1	hypothetical protein	-
CyaNS01_RS10110	1947554..1948198	+	645	WP_186696974.1	Uma2 family endonuclease	-
CyaNS01_RS10115	1948381..1948581	-	201	WP_186700635.1	class I SAM-dependent methyltransferase	-
CyaNS01_RS10120	1948592..1948999	-	408	WP_186696975.1	hypothetical protein	-
CyaNS01_RS10125	1949069..1949308	-	240	WP_186696976.1	Nif11-like leader peptide family natural product precursor	-
CyaNS01_RS10130	1949708..1950049	-	342	WP_186696977.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CyaNS01_RS10135	1950074..1950292	-	219	WP_186696978.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CyaNS01_RS10140	1950679..1950903	-	225	WP_186696979.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CyaNS01_RS10145	1950907..1952085	-	1179	WP_186696980.1	glycosyltransferase family 61 protein	-
CyaNS01_RS10150	1952108..1954015	-	1908	WP_186696981.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-109)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12463.63 Da
• Isoelectric Point: 10.4500

>T148559 WP_186696977.1 NZ_CP047940:c1950049-1949708 [Cyanobium sp. NS01]
MTPFDRFAGVRVPFPFSDREDSKRRPALVLSPPGFQQRCGHLVLAMITSARQSAWPLDWPIADLQAAGLKQPCLVRFKLF
TLDARLVLGAVGALEAEDQAGVMAHLRRLLPVA

Nucleotide

• Length: 342 bp

>T148559 NZ_CP063774:c3438457-3438200 [Escherichia coli O25b:H4-ST131]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7737.68 Da
• Isoelectric Point: 4.0385

>AT148559 WP_186696978.1 NZ_CP047940:c1950292-1950074 [Cyanobium sp. NS01]
MELATLTAKGQVTVPKAVRDALGLQQGDQLSWELEDGAVRAVAPLDVAYLKGLEATLAEWSSQADHEAFQDL

Nucleotide

• Length: 219 bp

>AT148559 NZ_CP063774:c3438188-3437637 [Escherichia coli O25b:H4-ST131]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA