Detailed information of TA system TA148547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(antitoxin)
• Location: 1449558..1450144
• Replicon: chromosome
• Accession: NZ_CP047938
• Organism: Synechococcus sp. A15-44

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SynA1544_RS08160
• Protein ID: WP_186515508.1
• Coordinates: 1449887..1450144 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SynA1544_RS08155
• Protein ID: WP_186512906.1
• Coordinates: 1449558..1449893 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SynA1544_RS08115	1445654..1445914	-	261	WP_186512900.1	hypothetical protein	-
SynA1544_RS08120	1445958..1446359	-	402	WP_186512901.1	helix-turn-helix domain-containing protein	-
SynA1544_RS08125	1446368..1446631	-	264	WP_186515506.1	type II toxin-antitoxin system HigB family toxin	-
SynA1544_RS08130	1446758..1446970	-	213	WP_186512902.1	type II toxin-antitoxin system HicA family toxin	-
SynA1544_RS08135	1446970..1447167	-	198	WP_186512903.1	type II toxin-antitoxin system HicB family antitoxin	-
SynA1544_RS08140	1447401..1447673	+	273	WP_186515507.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
SynA1544_RS08145	1447670..1448095	+	426	WP_186512905.1	type II toxin-antitoxin system VapC family toxin	-
SynA1544_RS08150	1448483..1449448	-	966	Protein_1586	DNA recombination protein RmuC	-
SynA1544_RS08155	1449558..1449893	-	336	WP_186512906.1	type II toxin-antitoxin system PemK/MazF family toxin	Antitoxin
SynA1544_RS08160	1449887..1450144	-	258	WP_186515508.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Toxin
SynA1544_RS08165	1450614..1450991	-	378	WP_186512907.1	nucleotidyltransferase domain-containing protein	-
SynA1544_RS08170	1451175..1451378	-	204	WP_186512908.1	hypothetical protein	-
SynA1544_RS08175	1451595..1452800	-	1206	WP_186512909.1	Fic family protein	-
SynA1544_RS08180	1453016..1453949	+	934	Protein_1592	IS481 family transposase	-
SynA1544_RS08185	1453919..1454464	-	546	WP_186512910.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• mRNA interferase PemK-like (9-109)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9171.42 Da
• Isoelectric Point: 4.4621

>T148547 WP_186515508.1 NZ_CP047938:c1450144-1449887 [Synechococcus sp. A15-44]
MQGVAVVRAKLVKIGNSRGIRLAKPLLEVAGLADEVEIEAAPGVLTIRPSAHPRAGWAEAASSFEPEGLLDEMSATRFDD
EEWSW

Nucleotide

• Length: 258 bp

>T148547 NZ_CP063774:c788566-788315 [Escherichia coli O25b:H4-ST131]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12348.30 Da
• Isoelectric Point: 8.5325

>AT148547 WP_186512906.1 NZ_CP047938:c1449893-1449558 [Synechococcus sp. A15-44]
MVTEEHVSRGDIFLVALNPTRGSEIRKTRPCVVVSPDELNAYLRTFIVAPLTTGGHPYPFRVRCKFDGKEGHVAPDQLRA
VDRDRLVKRLGSLSETTLTELLGVLQAMFAV

Nucleotide

• Length: 336 bp

>AT148547 NZ_CP063774:c788318-788064 [Escherichia coli O25b:H4-ST131]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA