Detailed information of TA system TA148539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3629138..3630159
• Replicon: chromosome
• Accession: NZ_CP047929
• Organism: Proteus mirabilis strain ChSC1905

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: GV833_RS16880
• Protein ID: WP_017827532.1
• Coordinates: 3629138..3629707 (-)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: GV833_RS16885
• Protein ID: WP_004245528.1
• Coordinates: 3629707..3630159 (-)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GV833_RS16875	3626668..3629022	-	2355	WP_036895567.1	DNA polymerase II	-
GV833_RS16880	3629138..3629707	-	570	WP_017827532.1	PIN domain-containing protein	Toxin
GV833_RS16885	3629707..3630159	-	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
GV833_RS16890	3630287..3630988	-	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
GV833_RS16895	3630981..3632561	-	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
GV833_RS16900	3632561..3633574	-	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
GV833_RS16905	3633843..3634373	+	531	WP_004245524.1	RNA pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T148539 WP_017827532.1 NZ_CP047929:c3629707-3629138 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T148539 NZ_CP063773:3127266-3127580 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
ATGCAATTCACTGTATACGGTAATACTGGGAAAAGCGTCGTTTACCCGCTGCTGCTCGATGTCACGAGCGATATTATTGG
ACAATTGAATCGTCGGATAGTGATCCCATTGCTCCCTATTGAAAAGTATCCGGCAGGCCTCCGACCGGATCGCCTTGTCC
CCGTCGTCATGCTGACGGACGGCAAAGAATACGCCGTAATGACCCACGAGCTGGCAAGTATCCCTGTCCAGACCCTGGGT
GCGGTGTTTTGTGATGCGTCGCAGTACCGTACTCAGGTAAAGGCTGCAATAGACTTCCTCATCGACGGGTTCTGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT148539 WP_004245528.1 NZ_CP047929:c3630159-3629707 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT148539 NZ_CP063773:3127027-3127263 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACGGTGGATCGTGCCTTACTGGTAAGAGCGCGTGAAGCGGG
TATTAACCTGAGTGCTACTCTGTCAACCGCACTGGATGCGGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAAA
ACAGGGAGGCTATCGACGCATTAAATCGTTTTCATGATGAAAACGGCTGTTTCAGTGATGAATACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3