Detailed information of TA system TA148534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2724766..2725295
• Replicon: chromosome
• Accession: NZ_CP047924
• Organism: Staphylococcus aureus strain SR130

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GWG03_RS13435
• Protein ID: WP_000621175.1
• Coordinates: 2724933..2725295 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GWG03_RS13430
• Protein ID: WP_000948331.1
• Coordinates: 2724766..2724936 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG03_RS13400 (2719803)	2719803..2720363	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
GWG03_RS13405 (2720571)	2720571..2721050	+	480	WP_001287087.1	hypothetical protein	-
GWG03_RS13410 (2721043)	2721043..2722626	+	1584	WP_001294626.1	PH domain-containing protein	-
GWG03_RS13415 (2722613)	2722613..2723104	+	492	WP_001205912.1	PH domain-containing protein	-
GWG03_RS13420 (2723108)	2723108..2723467	+	360	WP_000581197.1	holo-ACP synthase	-
GWG03_RS13425 (2723533)	2723533..2724681	+	1149	WP_001281154.1	alanine racemase	-
GWG03_RS13430 (2724766)	2724766..2724936	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GWG03_RS13435 (2724933)	2724933..2725295	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GWG03_RS13440 (2725645)	2725645..2726646	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GWG03_RS13445 (2726765)	2726765..2727091	+	327	WP_001052491.1	anti-sigma factor antagonist	-
GWG03_RS13450 (2727093)	2727093..2727572	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
GWG03_RS13455 (2727547)	2727547..2728317	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148534 WP_000621175.1 NZ_CP047924:2724933-2725295 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148534 NZ_CP063773:c1904028-1903774 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
GTGAAGCTAATCTGGTCAGAAGAAGCATGGGAAGACTACCTGTACTGGCAGGACATCGATAAGCGGATGGTCAAAAAGAT
CAATGAATTAATAAAAGAAACGCGTAGAACACCTTTTGAGGGAAAAGGAAAACCGGAGCCACTTAAACATAACCTCGCTG
GTTTCTGGTCGCGCCGTATAACCGAAGAGCATCGTCTGGTCTATGCCATCACCGATGACGCCATGATGATCGCTGCCTGC
CGCTACCACTATTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148534 WP_000948331.1 NZ_CP047924:2724766-2724936 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148534 NZ_CP063773:c1904276-1904025 [Pectobacterium carotovorum subsp. carotovorum PCCS1]
ATGCGTACAATTAGCTACAGCGAAGCCCGACAGAATCTATCGGCGACCATGATGAAAACGGTCGAAGACCGTGCTCCCAT
TCTTATCACCCGACAGAATGGTGAATCCTGCGTGCTGATGTCTCTGGAAGAGTATAATTCTCTAGAGGAAACCGCGTATT
TACTGCGTTCACCAGCAAATGCAAAAAGACTGATGAACTCAATCGAGAGCCTTAAGGCTGGCAATGGCGAAGAAAGAGAT
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1