Detailed information of TA system TA148524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 1201501..1201978
• Replicon: chromosome
• Accession: NZ_CP047924
• Organism: Staphylococcus aureus strain SR130

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: T1Y7S2
• Locus tag: GWG03_RS06085
• Protein ID: WP_000052076.1
• Coordinates: 1201790..1201978 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: GWG03_RS06080
• Protein ID: WP_001059690.1
• Coordinates: 1201501..1201761 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG03_RS06045 (1196894)	1196894..1197610	-	717	WP_000150011.1	type I 3-dehydroquinate dehydratase	-
GWG03_RS06050 (1197758)	1197758..1198180	+	423	WP_103147915.1	organic hydroperoxide resistance protein	-
GWG03_RS06055 (1198579)	1198579..1199073	+	495	WP_000569884.1	GNAT family N-acetyltransferase	-
GWG03_RS06060 (1199228)	1199228..1199845	+	618	WP_000255551.1	LysE/ArgO family amino acid transporter	-
GWG03_RS06065 (1199918)	1199918..1200499	-	582	WP_001140693.1	histidine phosphatase family protein	-
GWG03_RS06070 (1200724)	1200724..1200960	+	237	WP_000349581.1	hypothetical protein	-
GWG03_RS06075 (1200957)	1200957..1201160	-	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
GWG03_RS06080 (1201501)	1201501..1201761	+	261	WP_001059690.1	hypothetical protein	Antitoxin
GWG03_RS06085 (1201790)	1201790..1201978	+	189	WP_000052076.1	hypothetical protein	Toxin
GWG03_RS06090 (1202166)	1202166..1202735	+	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
GWG03_RS06095 (1202823)	1202823..1203107	+	285	WP_001004399.1	hypothetical protein	-
GWG03_RS06100 (1203169)	1203169..1203387	+	219	WP_103147916.1	hypothetical protein	-
GWG03_RS06105 (1203879)	1203879..1204079	-	201	WP_001059082.1	cold-shock protein	-
GWG03_RS06110 (1204436)	1204436..1205122	-	687	WP_000141557.1	thermonuclease family protein	-
GWG03_RS06115 (1205463)	1205463..1205984	-	522	WP_031876376.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7142.82 Da
• Isoelectric Point: 9.9971

>T148524 WP_000052076.1 NZ_CP047924:1201790-1201978 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T148524 NZ_CP063771:c9460-9173 [Acinetobacter sp. Ac-14]
ATGGAACTGTATTTCGAATGGGATGAGGCTAAAAACCAAAAGAATCAGAAAAAACATGATGTATCTTTTGAAACGGCAAG
CCTCGTTTTTGAAGATCCTTTACGGATCTCAATCCAGGATCGACATACCAATGGTGAAGAACGTTGGCAAACGATCGGTA
TGGTTAAAGGTGTACTGATGTTATTGGTGGCTCATACAATCATTGACGATGATGACTGTGAGATCATACGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAACGCAATCAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT148524 WP_001059690.1 NZ_CP047924:1201501-1201761 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT148524 NZ_CP063771:c9186-8875 [Acinetobacter sp. Ac-14]
ATGAGCATGGTTAGATATACACGCAAAGAACTGAATGAAAAATTCAGTGACAAGCAAGATGCTGAAATTAAACGCTTGCT
TGCAAAAGGAACTGTACCAGATGAACAATTAGATCTATCTGACATTCCTGAGATTACCGACTGGAGTCATGCTGTTCGCC
ATGGGCAATTTTATCGTCCAGTGAAGCAACAAACATCTGTTCGTCTAGATGCAGATGTACTTGCATGGTTAAAGGCTCAA
GGCAAAGGCTATCAGACACGCATGAATAAAATTCTGCGTGAAGCAATGCTAGAAGATCTTAAGAATCATTGA

Structures

Toxin

• AlphaFold DB: A0A0E0VP18

Antitoxin

• AlphaFold DB: A0A0E0VN56