Detailed information of TA system TA148520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tsbAT/-
• Location: 122331..123107
• Replicon: chromosome
• Accession: NZ_CP047924
• Organism: Staphylococcus aureus strain SR130

Toxin (Protein)

• Gene name: tsbT
• Uniprot ID: X5E2E6
• Locus tag: GWG03_RS00785
• Protein ID: WP_000031108.1
• Coordinates: 122955..123107 (+)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: tsbA
• Uniprot ID: W8U4V4
• Locus tag: GWG03_RS00780
• Protein ID: WP_001251224.1
• Coordinates: 122331..122930 (+)
• Length: 200 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG03_RS00760 (118247)	118247..119704	+	1458	WP_000649908.1	ABC transporter permease subunit	-
GWG03_RS00765 (119697)	119697..120419	+	723	WP_000590809.1	amino acid ABC transporter ATP-binding protein	-
GWG03_RS00770 (120570)	120570..121697	+	1128	WP_000379980.1	tRNA epoxyqueuosine(34) reductase QueG	-
GWG03_RS00775 (121702)	121702..122172	+	471	WP_000181394.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
GWG03_RS00780 (122331)	122331..122930	+	600	WP_001251224.1	glucosamine-6-phosphate isomerase	Antitoxin
GWG03_RS00785 (122955)	122955..123107	+	153	WP_000031108.1	SAS053 family protein	Toxin
GWG03_RS00790 (123655)	123655..124050	+	396	WP_000901023.1	hypothetical protein	-
GWG03_RS00795 (124246)	124246..125631	+	1386	WP_000116228.1	class II fumarate hydratase	-
GWG03_RS00800 (126084)	126084..126905	-	822	WP_000669376.1	RluA family pseudouridine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Glucosamine-6-phosphate isomerase (124-196)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5936.28 Da
• Isoelectric Point: 3.8962

>T148520 WP_000031108.1 NZ_CP047924:122955-123107 [Staphylococcus aureus]
MSKDKDPKLNYHEEENSMVTDFEDLKELGKEMEQISDQNDQEKNSEEDSQ

Nucleotide

• Length: 153 bp

>T148520 NZ_CP063770:2798-3118 [Acinetobacter sp. Ac-14]
ATGTTATTCATTGAAACCAGTATTTTTACCAAGCAAATCAAAGAGCTTGTTACTGATGAAGAATATCGTCAACTCCAGCA
AGATCTATTGATACAACCTGATAAAGGAGACTTGATCAAAAATGGTGGTGGCATTCGTAAAGTCCGTTGTGCTCAGGGTA
ATAAAGGCAAAAGTGGCGGTATTCGTGTGATCTATTACTGGGTTACAGAGGATGATCAGATTTTTTTCCTAGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAGACCTCGATCCTGCGTCAATTAGTGAAGGAGCAATTTAATGGATA
A

Antitoxin

Protein

• Length: 200 a.a.
• Molecular weight: 22343.47 Da
• Isoelectric Point: 5.1445

>AT148520 WP_001251224.1 NZ_CP047924:122331-122930 [Staphylococcus aureus]
MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAPVLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGV
PAGQVYPIAYEKDAIELIADKIKTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA

Nucleotide

• Length: 600 bp

>AT148520 NZ_CP063770:3111-3383 [Acinetobacter sp. Ac-14]
ATGGATAACAACTTATTTGATGACCTCGTTACTTCCATCAAAGAAGCTGGAGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTAACAGAACTTGAATTGCCTGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCAGAGTTTGCTGCAC
GCTTACATATTAGCGCGAGAACTCTACAAAATTGGGAACAAGGTCGTCGCTATCCGACTGGCCCAGCAGCGACTTTGATT
CGTATTCTTGATACCAATCCAACTCTGATTTAG

Structures

Toxin

• AlphaFold DB: X5E2E6

Antitoxin

• AlphaFold DB: W8U4V4