Detailed information of TA system TA148516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2815745..2816274
• Replicon: chromosome
• Accession: NZ_CP047922
• Organism: Staphylococcus aureus strain SR231

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GWG04_RS13995
• Protein ID: WP_000621175.1
• Coordinates: 2815912..2816274 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: GWG04_RS13990
• Protein ID: WP_000948331.1
• Coordinates: 2815745..2815915 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG04_RS13960 (2810782)	2810782..2811342	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
GWG04_RS13965 (2811550)	2811550..2812029	+	480	WP_212563500.1	hypothetical protein	-
GWG04_RS13970 (2812022)	2812022..2813605	+	1584	WP_001294626.1	PH domain-containing protein	-
GWG04_RS13975 (2813592)	2813592..2814083	+	492	WP_001205912.1	PH domain-containing protein	-
GWG04_RS13980 (2814087)	2814087..2814446	+	360	WP_000581197.1	holo-ACP synthase	-
GWG04_RS13985 (2814512)	2814512..2815660	+	1149	WP_001281154.1	alanine racemase	-
GWG04_RS13990 (2815745)	2815745..2815915	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
GWG04_RS13995 (2815912)	2815912..2816274	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GWG04_RS14000 (2816624)	2816624..2817625	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
GWG04_RS14005 (2817744)	2817744..2818070	+	327	WP_001052491.1	anti-sigma factor antagonist	-
GWG04_RS14010 (2818072)	2818072..2818551	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
GWG04_RS14015 (2818526)	2818526..2819296	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148516 WP_000621175.1 NZ_CP047922:2815912-2816274 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148516 NZ_CP063768:c390107-389757 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148516 WP_000948331.1 NZ_CP047922:2815745-2815915 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148516 NZ_CP063768:c390394-390113 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACGACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1