Detailed information of TA system TA148498

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4618935..4619461
• Replicon: chromosome
• Accession: NZ_CP047898
• Organism: Pseudarthrobacter sp. YJ56

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: GU243_RS21775
• Protein ID: WP_160678263.1
• Coordinates: 4619135..4619461 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GU243_RS21770
• Protein ID: WP_160678261.1
• Coordinates: 4618935..4619138 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU243_RS21740	4615227..4615814	+	588	WP_160678255.1	TetR family transcriptional regulator	-
GU243_RS21745	4615857..4616957	-	1101	WP_201762341.1	Gfo/Idh/MocA family oxidoreductase	-
GU243_RS21750	4617212..4617412	+	201	Protein_4300	2,3-butanediol dehydrogenase	-
GU243_RS21755	4617431..4617973	-	543	WP_160678257.1	hypothetical protein	-
GU243_RS21760	4617963..4618508	-	546	WP_160678259.1	hypothetical protein	-
GU243_RS21765	4618608..4618799	-	192	Protein_4303	FAD-dependent oxidoreductase	-
GU243_RS21770	4618935..4619138	+	204	WP_160678261.1	antitoxin MazE family protein	Antitoxin
GU243_RS21775	4619135..4619461	+	327	WP_160678263.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GU243_RS21780	4619565..4622221	+	2657	Protein_4306	magnesium-translocating P-type ATPase	-
GU243_RS21785	4622199..4622735	-	537	WP_160678265.1	HipA domain-containing protein	-
GU243_RS21790	4622845..4623102	-	258	WP_160678267.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4615857..4646540	30683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-106)

Antitoxin

• Antitoxin MazE-like (5-65)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11759.59 Da
• Isoelectric Point: 7.2038

>T148498 WP_160678263.1 NZ_CP047898:4619135-4619461 [Pseudarthrobacter sp. YJ56]
VNRGELWTVSGGTYAQKPRHALIIQDDLFAESDSITLLPLTSHLTDAPLLRLTVEPGQLTGLERVSQIMVDKLTTVRRAN
LGQRIGRIESATMVAVEQSLAVLLGLGR

Nucleotide

• Length: 327 bp

>T148498 NZ_CP063759:355416-355763 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7963.80 Da
• Isoelectric Point: 5.1171

>AT148498 WP_160678261.1 NZ_CP047898:4618935-4619138 [Pseudarthrobacter sp. YJ56]
MSVKDRVARHRAAMRERGFRQIQMWVPDARTEEFQREARRQSLAVATSDRAGDDQDFIEQISEDWPE

Nucleotide

• Length: 204 bp

>AT148498 NZ_CP063759:355763-356062 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA