Detailed information of TA system TA148493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1103342..1104576
• Replicon: chromosome
• Accession: NZ_CP047883
• Organism: Streptococcus mitis strain S022-V3-A4

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: M594_RS05500
• Protein ID: WP_173876178.1
• Coordinates: 1103818..1104576 (+)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: M594_RS05495
• Protein ID: WP_173876177.1
• Coordinates: 1103342..1103818 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M594_RS05455	1098889..1099122	+	234	WP_002933293.1	hypothetical protein	-
M594_RS05460	1099119..1099502	+	384	WP_173876171.1	chemotaxis protein	-
M594_RS05465	1099540..1099800	+	261	WP_173876172.1	hypothetical protein	-
M594_RS05470	1099806..1100342	+	537	Protein_1027	DUF3990 domain-containing protein	-
M594_RS05475	1100668..1100931	+	264	WP_173876173.1	hypothetical protein	-
M594_RS05480	1100964..1101551	+	588	WP_173876174.1	ABC transporter ATP-binding protein	-
M594_RS05485	1101555..1102739	+	1185	WP_173876175.1	hypothetical protein	-
M594_RS05490	1102962..1103189	+	228	WP_173876176.1	hypothetical protein	-
M594_RS05495	1103342..1103818	+	477	WP_173876177.1	helix-turn-helix domain-containing protein	Antitoxin
M594_RS05500	1103818..1104576	+	759	WP_173876178.1	zeta toxin family protein	Toxin
M594_RS05505	1104965..1105861	-	897	WP_173876179.1	tyrosine-type recombinase/integrase	-
M594_RS05510	1105953..1108232	+	2280	WP_173876180.1	ATP-binding protein	-
M594_RS05515	1108216..1109457	-	1242	WP_050088028.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1063162..1145716	82554

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 28754.61 Da
• Isoelectric Point: 6.4139

>T148493 WP_173876178.1 NZ_CP047883:1103818-1104576 [Streptococcus mitis]
MGIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTELSHLGYNLLIEGTLRTIDVPKKTAQLLKSKGYEVQLAIIATKPKLSYLSTLIRYEE
LYAINPNQACATPKEHHDLIVNNLVENTHQLEQLGIFEQIQIYQRDRTCVYDSRDDEISAAAVLHELLFGEWSQVEKDML
KSEEERFKDLTN

Nucleotide

• Length: 759 bp

>T148493 NZ_CP063747:c5242305-5241922 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18315.69 Da
• Isoelectric Point: 4.4354

>AT148493 WP_173876177.1 NZ_CP047883:1103342-1103818 [Streptococcus mitis]
MIGKNIKSLRKTHDLTQHEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVD

Nucleotide

• Length: 477 bp

>AT148493 NZ_CP063747:c5242547-5242305 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA