Detailed information of TA system TA148492

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1000045..1001279
• Replicon: chromosome
• Accession: NZ_CP047883
• Organism: Streptococcus mitis strain S022-V3-A4

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: M594_RS04960
• Protein ID: WP_173876117.1
• Coordinates: 1000521..1001279 (+)
• Length: 253 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: M594_RS04955
• Protein ID: WP_000579619.1
• Coordinates: 1000045..1000521 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M594_RS04905	995279..995563	+	285	WP_000969641.1	hypothetical protein	-
M594_RS04910	995577..995873	+	297	WP_000998891.1	hypothetical protein	-
M594_RS04915	995887..996084	-	198	WP_004249782.1	hypothetical protein	-
M594_RS04920	996244..996885	+	642	WP_000933082.1	hypothetical protein	-
M594_RS04925	996895..997980	+	1086	WP_173876116.1	toprim domain-containing protein	-
M594_RS04930	998031..998264	+	234	WP_000152419.1	hypothetical protein	-
M594_RS04935	998261..998644	+	384	WP_000159216.1	hypothetical protein	-
M594_RS04940	998685..998960	+	276	WP_000495442.1	hypothetical protein	-
M594_RS04945	999042..999674	+	633	WP_000424174.1	DUF3990 domain-containing protein	-
M594_RS04950	999665..999892	+	228	WP_000301902.1	hypothetical protein	-
M594_RS04955	1000045..1000521	+	477	WP_000579619.1	helix-turn-helix domain-containing protein	Antitoxin
M594_RS04960	1000521..1001279	+	759	WP_173876117.1	zeta toxin family protein	Toxin
M594_RS04965	1001675..1002571	-	897	WP_050088029.1	tyrosine-type recombinase/integrase	-
M594_RS04970	1002662..1003303	+	642	Protein_927	glycine hydroxymethyltransferase	-
M594_RS04975	1003303..1003704	+	402	Protein_928	C40 family peptidase	-
M594_RS04980	1003701..1004633	+	933	WP_001224318.1	conjugal transfer protein	-
M594_RS04985	1004878..1004994	+	117	WP_001814923.1	tetracycline resistance determinant leader peptide	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	mef(A) / msr(D) / tet(M)	-	963597..1027056	63459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 253 a.a.
• Molecular weight: 29058.03 Da
• Isoelectric Point: 7.5460

>T148492 WP_173876117.1 NZ_CP047883:1000521-1001279 [Streptococcus mitis]
VEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVESLVRELSHLGYNLLIEGTLRTIDVPKKTAQLLKSRGYEIQLALIATKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRGCVYDSREDKISAADILQELLFGEWSQVEKEML
KSGEERFKDLTN

Nucleotide

• Length: 759 bp

>T148492 NZ_CP063747:c4918789-4917872 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGTGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18231.66 Da
• Isoelectric Point: 4.4188

>AT148492 WP_000579619.1 NZ_CP047883:1000045-1000521 [Streptococcus mitis]
MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT148492 NZ_CP063747:c4919553-4918795 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA