Detailed information of TA system TA148484

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 2033..2761
• Replicon: plasmid pLD22-1-6kb
• Accession: NZ_CP047879
• Organism: Escherichia coli strain LD22-1

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: A0A8B6BBN5
• Locus tag: GUU87_RS24950
• Protein ID: WP_000675351.1
• Coordinates: 2033..2548 (-)
• Length: 172 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	2550..2761 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU87_RS24940	1..6430	-	6430	WP_001041636.1	Rop family plasmid primer RNA-binding protein	-
GUU87_RS24945	909..1619	-	711	WP_000967645.1	hypothetical protein	-
GUU87_RS24950	2033..2548	-	516	WP_000675351.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
-	2550..2761	-	212	NuclAT_0	-	Antitoxin
-	2550..2761	-	212	NuclAT_0	-	Antitoxin
-	2550..2761	-	212	NuclAT_0	-	Antitoxin
-	2550..2761	-	212	NuclAT_0	-	Antitoxin
-	2598..2690	-	93	NuclAT_5	-	-
-	2598..2690	-	93	NuclAT_5	-	-
-	2598..2690	-	93	NuclAT_5	-	-
-	2598..2690	-	93	NuclAT_5	-	-
-	2598..2725	-	128	NuclAT_3	-	-
-	2598..2725	-	128	NuclAT_3	-	-
-	2598..2725	-	128	NuclAT_3	-	-
-	2598..2725	-	128	NuclAT_3	-	-
-	2598..2762	-	165	NuclAT_1	-	-
-	2598..2762	-	165	NuclAT_1	-	-
-	2598..2762	-	165	NuclAT_1	-	-
-	2598..2762	-	165	NuclAT_1	-	-
-	2634..2761	-	128	NuclAT_2	-	-
-	2634..2761	-	128	NuclAT_2	-	-
-	2634..2761	-	128	NuclAT_2	-	-
-	2634..2761	-	128	NuclAT_2	-	-
-	2669..2761	-	93	NuclAT_4	-	-
-	2669..2761	-	93	NuclAT_4	-	-
-	2669..2761	-	93	NuclAT_4	-	-
-	2669..2761	-	93	NuclAT_4	-	-
-	2705..2761	-	57	NuclAT_6	-	-
-	2705..2761	-	57	NuclAT_6	-	-
-	2705..2761	-	57	NuclAT_6	-	-
-	2705..2761	-	57	NuclAT_6	-	-
GUU87_RS24955	3854..4438	+	585	Protein_3	primase C-terminal domain-containing protein	-
GUU87_RS24960	4435..6114	+	1680	WP_001774712.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..6430	6430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (1-159)

Sequences

Toxin

Protein

• Length: 172 a.a.
• Molecular weight: 19724.66 Da
• Isoelectric Point: 6.7273

>T148484 WP_000675351.1 NZ_CP047879:c2548-2033 [Escherichia coli]
MKFYTISSRYIEYLKGFEDKVPNSEDPNYQNPKAFIGIVLEIQGHKYLAPLTSPKPWHSTIKESSPSFFKLHENGVPENQ
LGLINLKFMIPIIEAEVSLLDLNGMPNTAYKRMLYKQLQFIRVNSDKIAEKSELLRKLVLQGKMQGTCNFSLLEEKYRDF
GKEANDTEEGE

Nucleotide

• Length: 516 bp

>T148484 NZ_CP063747:350588-350935 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Nucleotide

• Length: 212 bp

>AT148484 NZ_CP047879:c2761-2550 [Escherichia coli]
AGGTGATTTGCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTAAGTGCAGCTAGAAATTCAGGTGATTT
GCTACCTTTAAGTGCAGCTAGAAATTCAGGTGATTTGCTACCTTAAGTGCAGCTAGAAATTTAGGTCATTTACTACCTTA
AAGTAATACAGTAAAAGAGGCGACTACACAGTCGCCTTTTTTACGAGAAAAA

Structures

Toxin

• AlphaFold DB: A0A8B6BBN5

Antitoxin