Detailed information of TA system TA148475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 4123825..4125160
• Replicon: chromosome
• Accession: NZ_CP047876
• Organism: Escherichia coli strain LD22-1

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: F4VMR9
• Locus tag: GUU87_RS19850
• Protein ID: WP_000342497.1
• Coordinates: 4123825..4124772 (-)
• Length: 316 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A228Z6F8
• Locus tag: GUU87_RS19855
• Protein ID: WP_000152739.1
• Coordinates: 4124765..4125160 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU87_RS19825	4119678..4120394	+	717	WP_001295734.1	N-acetylneuraminic acid outer membrane channel NanC	-
GUU87_RS19830	4120414..4121520	+	1107	WP_001298927.1	N-acetylneuraminate epimerase	-
GUU87_RS19835	4121585..4122565	+	981	WP_000991446.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GUU87_RS19840	4122573..4123223	-	651	WP_001037963.1	HNH endonuclease	-
GUU87_RS19845	4123602..4123787	+	186	WP_000066585.1	hypothetical protein	-
GUU87_RS19850	4123825..4124772	-	948	WP_000342497.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GUU87_RS19855	4124765..4125160	-	396	WP_000152739.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
GUU87_RS19860	4125230..4126063	-	834	WP_001192741.1	DUF4942 domain-containing protein	-
GUU87_RS19865	4126369..4127925	+	1557	WP_000202816.1	type I restriction-modification system subunit M	-
GUU87_RS19870	4127922..4129088	+	1167	WP_001293040.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	4114151..4146816	32665

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-303)

Antitoxin

• Protein of unknown function DUF6088 (7-108)

Sequences

Toxin

Protein

• Length: 316 a.a.
• Molecular weight: 35588.79 Da
• Isoelectric Point: 4.9737

>T148475 WP_000342497.1 NZ_CP047876:c4124772-4123825 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Nucleotide

• Length: 948 bp

>T148475 NZ_CP063709:c56770-56465 [Salmonella enterica subsp. enterica serovar Enteritidis]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14605.90 Da
• Isoelectric Point: 11.6915

>AT148475 WP_000152739.1 NZ_CP047876:c4125160-4124765 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Nucleotide

• Length: 396 bp

>AT148475 NZ_CP063709:c56990-56772 [Salmonella enterica subsp. enterica serovar Enteritidis]
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT

Structures

Toxin

• AlphaFold DB: A0A1L5BAQ6

Antitoxin

• AlphaFold DB: A0A228Z6F8