Detailed information of TA system TA148470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2403490..2404128
• Replicon: chromosome
• Accession: NZ_CP047876
• Organism: Escherichia coli strain LD22-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GUU87_RS11480
• Protein ID: WP_000813794.1
• Coordinates: 2403952..2404128 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GUU87_RS11475
• Protein ID: WP_001270286.1
• Coordinates: 2403490..2403906 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU87_RS11455	2398642..2399583	-	942	WP_001251319.1	ABC transporter permease	-
GUU87_RS11460	2399584..2400597	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
GUU87_RS11465	2400615..2401760	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
GUU87_RS11470	2402005..2403411	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
GUU87_RS11475	2403490..2403906	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GUU87_RS11480	2403952..2404128	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GUU87_RS11485	2404350..2404580	+	231	WP_000494244.1	YncJ family protein	-
GUU87_RS11490	2404672..2406633	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GUU87_RS11495	2406706..2407242	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
GUU87_RS11500	2407295..2408509	+	1215	WP_089078769.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2408549..2409814	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T148470 WP_000813794.1 NZ_CP047876:c2404128-2403952 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T148470 NZ_CP063687:c1179573-1179223 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT148470 WP_001270286.1 NZ_CP047876:c2403906-2403490 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT148470 NZ_CP063687:c1179860-1179579 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 6HPB