Detailed information of TA system TA148459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1337845..1338470
• Replicon: chromosome
• Accession: NZ_CP047876
• Organism: Escherichia coli strain LD22-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GUU87_RS06470
• Protein ID: WP_000911330.1
• Coordinates: 1338072..1338470 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: GUU87_RS06465
• Protein ID: WP_000450524.1
• Coordinates: 1337845..1338072 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU87_RS06440	1333648..1334118	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
GUU87_RS06445	1334118..1334690	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
GUU87_RS06450	1334836..1335714	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GUU87_RS06455	1335731..1336765	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
GUU87_RS06460	1336978..1337691	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
GUU87_RS06465	1337845..1338072	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
GUU87_RS06470	1338072..1338470	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GUU87_RS06475	1338617..1339480	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
GUU87_RS06480	1339495..1341510	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
GUU87_RS06485	1341584..1342282	+	699	WP_000679823.1	esterase	-
GUU87_RS06490	1342392..1342592	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T148459 WP_000911330.1 NZ_CP047876:1338072-1338470 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T148459 NZ_CP063659:c2100370-2100239 [Synechocystis sp. PCC 7339]
ATTGGAAAAACTCTTCAAGGTTTGAATCAAACTATTATTGATAACCGAGTGGCCATTTTATTCAGTGAAGATTTGTTTGC
TGAACTGATGGAGGTATTACACCGTCCTAAGTTCAAGAAATATTTTTCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT148459 WP_000450524.1 NZ_CP047876:1337845-1338072 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT148459 NZ_CP063659:c2100635-2100417 [Synechocystis sp. PCC 7339]
ATGAAATCATTATCATCACTAGACTTTCTTGAGCTGAAAAATATTATTGCCCAATGTAATCTTGAAGAAAAACTAGAATT
ACTAGAACTTTTAAAGAAGGATACTTTTGCCACCAGATTCAACAAATTTCTCAATTCCGTCAAAACCGATGAATTAACTC
TAGAAGACATTACTCGAGAAGTTGAAGCAGTCCGGCAAGCTAATTACCATAAGCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33