148458

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1044696..1045279 Replicon chromosome
Accession NZ_CP047876
Organism Escherichia coli strain LD22-1

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag GUU87_RS05040 Protein ID WP_000254745.1
Coordinates 1044944..1045279 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag GUU87_RS05035 Protein ID WP_000581937.1
Coordinates 1044696..1044944 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU87_RS05025 1041035..1042336 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
GUU87_RS05030 1042384..1044618 + 2235 WP_000226815.1 GTP pyrophosphokinase -
GUU87_RS05035 1044696..1044944 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
GUU87_RS05040 1044944..1045279 + 336 WP_000254745.1 endoribonuclease MazF Toxin
GUU87_RS05045 1045350..1046141 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
GUU87_RS05050 1046369..1048006 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
GUU87_RS05055 1048094..1049392 + 1299 WP_000036723.1 phosphopyruvate hydratase -
GUU87_RS05060 1049448..1049810 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T148458 WP_000254745.1 NZ_CP047876:1044944-1045279 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T148458 NZ_CP063659:c1938572-1938303 [Synechocystis sp. PCC 7339]
ATGAGATACTTGGTTTTTGAAGGAAATACTTGGGAAACTTACGAAGAGCTTCGGCAGAAGGATAAAATTTTGCACAAAAA
TCTCTGTAAACTTATTAAAGAAATGCTGAGAGGAGATCCCGCCAAAGGTACCGGAAAACCTGAGCCACTCAAGCACAATT
TGTCGGGATTATGGTCAAGACGCATTTCCCAGAAAGACCGTCTTATCTATAAGTTTGACGACAAATATATTTACATTTTT
GCCATTGGTGGCCATTACAATGAGTACTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT148458 WP_000581937.1 NZ_CP047876:1044696-1044944 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT148458 NZ_CP063659:c1938180-1937953 [Synechocystis sp. PCC 7339]
ATGAACACCGCTCAAATTAGCACTGATGGCACCCATCAAATCATAATCTTACCAGAAAATTTTACAATAGCCGGAAGCGA
GGTTTACATTAAAAAAATTGGAAGTACGATTATTTTGATTGCCAAAGATAACCCTTGGCAATCCTTAATTGAAAGCTTAG
ATCAATTTTCAGATGACTTTATGGCAACTAGGGAACAACCACCCCTTGATGTGCGAGAAGAATTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References