Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 908354..909008 Replicon chromosome
Accession NZ_CP047876
Organism Escherichia coli strain LD22-1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag GUU87_RS04420 Protein ID WP_000244772.1
Coordinates 908601..909008 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GUU87_RS04415 Protein ID WP_000354046.1
Coordinates 908354..908620 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUU87_RS04390 903642..904385 + 744 WP_032220047.1 SDR family oxidoreductase -
GUU87_RS04395 904442..905875 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
GUU87_RS04400 905920..906231 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
GUU87_RS04405 906395..907054 + 660 WP_000250274.1 hemolysin III family protein -
GUU87_RS04410 907131..908111 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GUU87_RS04415 908354..908620 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GUU87_RS04420 908601..909008 + 408 WP_000244772.1 protein YgfX Toxin
GUU87_RS04425 909048..909569 - 522 WP_001055874.1 flavodoxin FldB -
GUU87_RS04430 909681..910577 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GUU87_RS04435 910602..911312 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GUU87_RS04440 911318..913051 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T148457 WP_000244772.1 NZ_CP047876:908601-909008 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T148457 NZ_CP063659:c1937889-1937551 [Synechocystis sp. PCC 7339]
TTGGCAAAACTTCAAGCCCTGGAGGTGTCAGAGGTGGGTATTTCATCCATTACCCTGGCAGAATTAGAATATGGTGTTAG
CAAAAGTCAACGGCAGTCGAAAAATCGTGATGCCTTAATGCAATTCTTGTTGCCGCTAGAAATCGTGGAATTTAATCAGG
AAGCGGCGATCGCCTATGGCAACATTAGAAGTAATCTAGAAAGTAGAGGTCTTGTCATTGGGGCTATGGATATGCTGATT
GCGGCCCATGCGTTAAGTCTGGGAGTAACTCTTGTGAGCAATAATGTTCGAGAATTTTCACGCATTAACAATTTATCCTT
GGAAAACTGGGCAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT148457 WP_000354046.1 NZ_CP047876:908354-908620 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT148457 NZ_CP063659:c1938180-1937953 [Synechocystis sp. PCC 7339]
ATGAACACCGCTCAAATTAGCACTGATGGCACCCATCAAATCATAATCTTACCAGAAAATTTTACAATAGCCGGAAGCGA
GGTTTACATTAAAAAAATTGGAAGTACGATTATTTTGATTGCCAAAGATAACCCTTGGCAATCCTTAATTGAAAGCTTAG
ATCAATTTTCAGATGACTTTATGGCAACTAGGGAACAACCACCCCTTGATGTGCGAGAAGAATTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References