Detailed information of TA system TA148453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2748368..2748897
• Replicon: chromosome
• Accession: NZ_CP047867
• Organism: Staphylococcus aureus strain UP_419

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S59_RS13555
• Protein ID: WP_160195537.1
• Coordinates: 2748535..2748897 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S59_RS13550
• Protein ID: WP_000948331.1
• Coordinates: 2748368..2748538 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S59_RS13520	2743405..2743965	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S59_RS13525	2744173..2744652	+	480	WP_001287087.1	hypothetical protein	-
E3S59_RS13530	2744645..2746228	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S59_RS13535	2746215..2746706	+	492	WP_001205912.1	PH domain-containing protein	-
E3S59_RS13540	2746710..2747069	+	360	WP_000581197.1	holo-ACP synthase	-
E3S59_RS13545	2747135..2748283	+	1149	WP_001281154.1	alanine racemase	-
E3S59_RS13550	2748368..2748538	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S59_RS13555	2748535..2748897	+	363	WP_160195537.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S59_RS13560	2749246..2750247	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
E3S59_RS13565	2750366..2750692	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S59_RS13570	2750694..2751173	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S59_RS13575	2751148..2751918	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13383.65 Da
• Isoelectric Point: 10.2892

>T148453 WP_160195537.1 NZ_CP047867:2748535-2748897 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKGSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148453 NZ_CP063519:10968-11273 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTCATTGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCTATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148453 WP_000948331.1 NZ_CP047867:2748368-2748538 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148453 NZ_CP063519:10748-10966 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGCGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1