Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2727124..2727638 Replicon chromosome
Accession NZ_CP047867
Organism Staphylococcus aureus strain UP_419

Toxin (Protein)


Gene name TsdT Uniprot ID T1YA99
Locus tag E3S59_RS13460 Protein ID WP_000581792.1
Coordinates 2727429..2727638 (+) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag E3S59_RS13455 Protein ID WP_000138932.1
Coordinates 2727124..2727417 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3S59_RS13420 2722287..2722928 + 642 WP_000483153.1 thiamine phosphate synthase -
E3S59_RS13425 2723014..2723886 + 873 WP_000725802.1 membrane protein insertase YidC -
E3S59_RS13445 2724799..2725446 - 648 WP_001187630.1 HD domain-containing protein -
E3S59_RS13450 2725474..2726958 - 1485 WP_000571549.1 cardiolipin synthase -
E3S59_RS13455 2727124..2727417 + 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
E3S59_RS13460 2727429..2727638 + 210 WP_000581792.1 heavy-metal-associated domain-containing protein Toxin
E3S59_RS13465 2727766..2727903 + 138 WP_000828354.1 hypothetical protein -
E3S59_RS13470 2728167..2729369 - 1203 WP_001109939.1 rod shape-determining protein RodA -
E3S59_RS13475 2729687..2730757 + 1071 WP_000159631.1 D-alanine--D-alanine ligase -
E3S59_RS13480 2730772..2732130 + 1359 WP_000611464.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7843.77 Da        Isoelectric Point: 4.1008

>T148452 WP_000581792.1 NZ_CP047867:2727429-2727638 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Download         Length: 210 bp

>T148452 NZ_CP063518:c4382710-4382435 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT148452 WP_000138932.1 NZ_CP047867:2727124-2727417 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT148452 NZ_CP063518:c4382438-4382079 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FF1

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References