Detailed information of TA system TA148419

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2744967..2745496
• Replicon: chromosome
• Accession: NZ_CP047863
• Organism: Staphylococcus aureus strain UP_1106

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S63_RS13590
• Protein ID: WP_000621175.1
• Coordinates: 2745134..2745496 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S63_RS13585
• Protein ID: WP_000948331.1
• Coordinates: 2744967..2745137 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S63_RS13555	2740004..2740564	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S63_RS13560	2740772..2741251	+	480	WP_001287087.1	hypothetical protein	-
E3S63_RS13565	2741244..2742827	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S63_RS13570	2742814..2743305	+	492	WP_001205912.1	PH domain-containing protein	-
E3S63_RS13575	2743309..2743668	+	360	WP_000581197.1	holo-ACP synthase	-
E3S63_RS13580	2743734..2744882	+	1149	WP_001281154.1	alanine racemase	-
E3S63_RS13585	2744967..2745137	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S63_RS13590	2745134..2745496	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S63_RS13595	2745845..2746846	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
E3S63_RS13600	2746965..2747291	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S63_RS13605	2747293..2747772	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S63_RS13610	2747747..2748517	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148419 WP_000621175.1 NZ_CP047863:2745134-2745496 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148419 NZ_CP063515:3710514-3710891 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148419 WP_000948331.1 NZ_CP047863:2744967-2745137 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148419 NZ_CP063515:3710057-3710425 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCTCGTCTGGTGCAAGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1