Detailed information of TA system TA148418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2723723..2724237
• Replicon: chromosome
• Accession: NZ_CP047863
• Organism: Staphylococcus aureus strain UP_1106

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: E3S63_RS13495
• Protein ID: WP_000581792.1
• Coordinates: 2724028..2724237 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: E3S63_RS13490
• Protein ID: WP_000138932.1
• Coordinates: 2723723..2724016 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S63_RS13455	2718886..2719527	+	642	WP_000483153.1	thiamine phosphate synthase	-
E3S63_RS13460	2719613..2720485	+	873	WP_000725802.1	membrane protein insertase YidC	-
E3S63_RS13480	2721398..2722045	-	648	WP_001187630.1	HD domain-containing protein	-
E3S63_RS13485	2722073..2723557	-	1485	WP_000571549.1	cardiolipin synthase	-
E3S63_RS13490	2723723..2724016	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
E3S63_RS13495	2724028..2724237	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
E3S63_RS13500	2724365..2724502	+	138	WP_000828354.1	hypothetical protein	-
E3S63_RS13505	2724766..2725968	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
E3S63_RS13510	2726286..2727356	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
E3S63_RS13515	2727371..2728729	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T148418 WP_000581792.1 NZ_CP047863:2724028-2724237 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T148418 NZ_CP063515:3502349-3502522 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT148418 WP_000138932.1 NZ_CP047863:2723723-2724016 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT148418 NZ_CP063515:3502025-3502321 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCCGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13