Detailed information of TA system TA148385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2785941..2786470
• Replicon: chromosome
• Accession: NZ_CP047859
• Organism: Staphylococcus aureus strain UP_1405

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S65_RS13880
• Protein ID: WP_000621175.1
• Coordinates: 2786108..2786470 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S65_RS13875
• Protein ID: WP_000948331.1
• Coordinates: 2785941..2786111 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S65_RS13845	2780978..2781538	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S65_RS13850	2781746..2782225	+	480	WP_001287087.1	hypothetical protein	-
E3S65_RS13855	2782218..2783801	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S65_RS13860	2783788..2784279	+	492	WP_001205912.1	PH domain-containing protein	-
E3S65_RS13865	2784283..2784642	+	360	WP_000581197.1	holo-ACP synthase	-
E3S65_RS13870	2784708..2785856	+	1149	WP_001281154.1	alanine racemase	-
E3S65_RS13875	2785941..2786111	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S65_RS13880	2786108..2786470	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S65_RS13885	2786819..2787820	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
E3S65_RS13890	2787939..2788265	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S65_RS13895	2788267..2788746	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S65_RS13900	2788721..2789491	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148385 WP_000621175.1 NZ_CP047859:2786108-2786470 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148385 NZ_CP063511:c47083-46685 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148385 WP_000948331.1 NZ_CP047859:2785941-2786111 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148385 NZ_CP063511:c47379-47086 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1