Detailed information of TA system TA148384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsdAT/-
• Location: 2764697..2765211
• Replicon: chromosome
• Accession: NZ_CP047859
• Organism: Staphylococcus aureus strain UP_1405

Toxin (Protein)

• Gene name: TsdT
• Uniprot ID: T1YA99
• Locus tag: E3S65_RS13785
• Protein ID: WP_000581792.1
• Coordinates: 2765002..2765211 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: TsdA
• Uniprot ID: W8UW13
• Locus tag: E3S65_RS13780
• Protein ID: WP_000138932.1
• Coordinates: 2764697..2764990 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S65_RS13745	2759860..2760501	+	642	WP_000483153.1	thiamine phosphate synthase	-
E3S65_RS13750	2760587..2761459	+	873	WP_000725802.1	membrane protein insertase YidC	-
E3S65_RS13770	2762372..2763019	-	648	WP_001187630.1	HD domain-containing protein	-
E3S65_RS13775	2763047..2764531	-	1485	WP_000571549.1	cardiolipin synthase	-
E3S65_RS13780	2764697..2764990	+	294	WP_000138932.1	copper-sensing transcriptional repressor CsoR	Antitoxin
E3S65_RS13785	2765002..2765211	+	210	WP_000581792.1	heavy-metal-associated domain-containing protein	Toxin
E3S65_RS13790	2765339..2765476	+	138	WP_000828354.1	hypothetical protein	-
E3S65_RS13795	2765740..2766942	-	1203	WP_001109939.1	rod shape-determining protein RodA	-
E3S65_RS13800	2767260..2768330	+	1071	WP_000159631.1	D-alanine--D-alanine ligase	-
E3S65_RS13805	2768345..2769703	+	1359	WP_000611464.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Heavy metal-associated domain, HMA (23-66)

Antitoxin

• Metal-sensitive transcriptional repressor (14-92)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7843.77 Da
• Isoelectric Point: 4.1008

>T148384 WP_000581792.1 NZ_CP047859:2765002-2765211 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVF

Nucleotide

• Length: 210 bp

>T148384 NZ_CP063509:c18840-18559 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11197.82 Da
• Isoelectric Point: 6.2243

>AT148384 WP_000138932.1 NZ_CP047859:2764697-2764990 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Nucleotide

• Length: 294 bp

>AT148384 NZ_CP063509:c19081-18830 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• PDB: 6FF1

Antitoxin

• AlphaFold DB: W8UW13