Detailed information of TA system TA148365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2795379..2795908
• Replicon: chromosome
• Accession: NZ_CP047856
• Organism: Staphylococcus aureus strain UP_1435

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3S66_RS13935
• Protein ID: WP_000621175.1
• Coordinates: 2795546..2795908 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3S66_RS13930
• Protein ID: WP_000948331.1
• Coordinates: 2795379..2795549 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S66_RS13900	2790416..2790976	+	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-
E3S66_RS13905	2791184..2791663	+	480	WP_001287087.1	hypothetical protein	-
E3S66_RS13910	2791656..2793239	+	1584	WP_001294626.1	PH domain-containing protein	-
E3S66_RS13915	2793226..2793717	+	492	WP_001205912.1	PH domain-containing protein	-
E3S66_RS13920	2793721..2794080	+	360	WP_000581197.1	holo-ACP synthase	-
E3S66_RS13925	2794146..2795294	+	1149	WP_001281154.1	alanine racemase	-
E3S66_RS13930	2795379..2795549	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3S66_RS13935	2795546..2795908	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3S66_RS13940	2796257..2797258	+	1002	WP_000390827.1	PP2C family protein-serine/threonine phosphatase	-
E3S66_RS13945	2797377..2797703	+	327	WP_001052491.1	anti-sigma factor antagonist	-
E3S66_RS13950	2797705..2798184	+	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3S66_RS13955	2798159..2798929	+	771	WP_001041111.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T148365 WP_000621175.1 NZ_CP047856:2795546-2795908 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T148365 NZ_CP063503:2428361-2428537 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT148365 WP_000948331.1 NZ_CP047856:2795379-2795549 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT148365 NZ_CP063503:2428583-2428999 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1