Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /HTH_19(antitoxin)
Location 1745260..1746048 Replicon chromosome
Accession NZ_CP047856
Organism Staphylococcus aureus strain UP_1435

Toxin (Protein)


Gene name - Uniprot ID A0A2I7Y5B3
Locus tag E3S66_RS08905 Protein ID WP_000525004.1
Coordinates 1745587..1746048 (+) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0E7YIA0
Locus tag E3S66_RS08900 Protein ID WP_000333630.1
Coordinates 1745260..1745574 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3S66_RS08845 1740453..1741010 - 558 WP_000645045.1 DUF2815 family protein -
E3S66_RS08850 1741036..1742202 - 1167 WP_000762530.1 DUF2800 domain-containing protein -
E3S66_RS08855 1742199..1742561 - 363 WP_000985972.1 hypothetical protein -
E3S66_RS08860 1742576..1742899 - 324 WP_000174998.1 hypothetical protein -
E3S66_RS08865 1742978..1743139 - 162 WP_001285954.1 DUF1270 domain-containing protein -
E3S66_RS08870 1743152..1743415 - 264 WP_001124160.1 helix-turn-helix domain-containing protein -
E3S66_RS08875 1743440..1743655 - 216 WP_001097552.1 hypothetical protein -
E3S66_RS08880 1743711..1743920 + 210 WP_000642492.1 hypothetical protein -
E3S66_RS08885 1743910..1744053 - 144 WP_000939498.1 hypothetical protein -
E3S66_RS08890 1744082..1744858 - 777 WP_001148544.1 Rha family transcriptional regulator -
E3S66_RS08895 1744872..1745108 - 237 WP_001121027.1 helix-turn-helix domain-containing protein -
E3S66_RS08900 1745260..1745574 + 315 WP_000333630.1 helix-turn-helix domain-containing protein Antitoxin
E3S66_RS08905 1745587..1746048 + 462 WP_000525004.1 hypothetical protein Toxin
E3S66_RS08910 1746066..1746650 + 585 WP_000825948.1 hypothetical protein -
E3S66_RS08915 1746880..1747047 + 168 WP_000705242.1 hypothetical protein -
E3S66_RS08920 1747187..1747726 + 540 WP_117434767.1 type II toxin-antitoxin system PemK/MazF family toxin -
E3S66_RS08925 1747901..1749106 + 1206 WP_031883891.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - geh 1705020..1759679 54659


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(4-62)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 18074.46 Da        Isoelectric Point: 4.6915

>T148355 WP_000525004.1 NZ_CP047856:1745587-1746048 [Staphylococcus aureus]
MGLYEETLIQHDYIEIREADVLPDNLDGVWLGDLILIKRGLSDREKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFISAVPLREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T148355 NZ_CP063502:c12765-12478 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12063.56 Da        Isoelectric Point: 6.4736

>AT148355 WP_000333630.1 NZ_CP047856:1745260-1745574 [Staphylococcus aureus]
MCFSKRMKQSREKQGMTLAELGRKIGKTEATVQRYESGNIKNLKNDTIESIATALNVNPAYLMGWVEENDDEVQHRAAHL
EGELTDDEWQRVLDYADYIRSKRK

Download         Length: 315 bp

>AT148355 NZ_CP063502:c13013-12762 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAAGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I7Y5B3

Antitoxin

Source ID Structure
AlphaFold DB A0A0E7YIA0

References